Team:Warsaw/Calendar-Stage1/17 August 2010

From 2010.igem.org

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17.08.2010 Tuesday

Previous day: Set up liquid cultures of E.coli TOP10 with measured constructs [Ania O.]

We used a protocol described here

Testing the strenght of RBS [Ania O.,Cherry]

Preparation of samples by dilluting the bacteria cultures and examining them in flow cytometer (all according the protocol, but each RBS preformed from one independent culture)

Data analysis with Cell Quest and Microsoft Excel[Cherry]

Legend:

17-08-2010.001- TOP10 without construct
17-08-2010.015- J61100
17-08-2010.016- J61101
17-08-2010.017- J61107
17-08-2010.018- J61117
17-08-2010.019- J61127
17-08-2010.020- B0030
17-08-2010.021- B0031
17-08-2010.022- B0032
17-08-2010.023- B0033
17-08-2010.024- B0034

cytometer plots:
FSC- Forward Scatter, cell size
SSC- Side Scatter, cell complexity
FL-1- FL-1 sensor, cell fluorescence

For pdf version click here.


Cytometer data processed with Cell Quest:




Cell Quest data processed with Microsoft Excel:



geo mean- geometric mean of fluorescence of events in FL1
peak channel- nr of channel which recorded the biggest nr of events

partgeo meanpeak channel
allm1allm1
J611004,145,6416
J6110119,9834,77146
J6110720,5634,33145
J611175,9214,71111
J611277,3210,0619
B003099,78180,661254
B003143,566,73169
B003228,1546,28148
B003386,21152,421205
B003428,4841,65145
partgeo mean %B0033peak channel %B0033
allm1allm1
J611000,0480,03710,0293
J611010,23180,228110,2244
J611070,23850,225210,2195
J611170,06870,096510,0537
J611270,08490,06610,0439
B00301,15741,185311,239
B00310,50460,437810,3366
B00320,32650,303610,2341
B00331111
B00340,33040,273310,2195