Team:Warsaw/Calendar-Stage1/16 July 2010

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<br>CFP1
<br>CFP1
<br>CFP2
<br>CFP2
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<br><br><div class="note">Checking attendance of internal RBS within mOrange, mCherry, YFP [Kasia, Milena]</div>
 
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<br> Cloning: mOrange after J23100, mCherry after J23100, YFP after J23100 [promotor]:
 
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<br>  -> ligation (20ul of fluorescent protein and 2ul of vector with promotor): mOrange + J23100, mCherry + J23100, YFP + J23100; samples were after gel out (from 12.07.2010r), incubation over 1 hour;
 
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<br>  -> transformation: 10ul of ligation mix added to competent E. coli DH5alpha cells.
 
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<br> Unfortunately next day there was nothing on the plates after this transformation.
 
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<br><br><div class="note">mCHerry NLS transformation (using E. coli Top10)[Ania P.] -> uzup. </div>
 

Revision as of 13:03, 31 July 2010

Example Tabs


16.07.2010 Friday

Cloning CommunityRBSes-GFPterminator behind J23100 [promotor]

1. EcoRI/Pst digest of obtained clones to check size of the constructs [Kasia]
J23100-B0030-GFPterminator sample 1
J23100-B0031-GFPterminator sample 1
J23100-B0032-GFPterminator sample 1
J23100-B0033-GFPterminator sample 1
J23100-B0034-GFPterminator sample 1
2. GEL []
3. Set up liquid culture of ‘cloning intermediates’ communityRBSes-GFPterminator to prepare stocks [Cherry];
samples:
RBS+GFP 10.1 F zag
RBS+GFP 7.2G zag
RBS+GFP 11.1P zag
RBS+GFP 10.1F
RBS+GFP 11.1P
RBS+GFP 8.1E
RBS+GFP 9.1zag
RBS+GFP 9.1A
RBS+GFP 8.1Ezag
YFP1
YFP2
CFP1
CFP2

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