http://2010.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Stage1/11_July_2010&feed=atom&action=history
Team:Warsaw/Calendar-Stage1/11 July 2010 - Revision history
2024-03-28T17:28:08Z
Revision history for this page on the wiki
MediaWiki 1.16.5
http://2010.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Stage1/11_July_2010&diff=195155&oldid=prev
Mlower: Team:Warsaw/Calendar-Stage4/12 July 2010 moved to Team:Warsaw/Calendar-Stage1/11 July 2010
2010-10-27T20:27:03Z
<p><a href="/Team:Warsaw/Calendar-Stage4/12_July_2010" class="mw-redirect" title="Team:Warsaw/Calendar-Stage4/12 July 2010">Team:Warsaw/Calendar-Stage4/12 July 2010</a> moved to <a href="/Team:Warsaw/Calendar-Stage1/11_July_2010" title="Team:Warsaw/Calendar-Stage1/11 July 2010">Team:Warsaw/Calendar-Stage1/11 July 2010</a></p>
<table style="background-color: white; color:black;">
<tr valign='top'>
<td colspan='1' style="background-color: white; color:black;">← Older revision</td>
<td colspan='1' style="background-color: white; color:black;">Revision as of 20:27, 27 October 2010</td>
</tr></table>
Mlower
http://2010.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Stage1/11_July_2010&diff=27813&oldid=prev
Olchowik at 01:32, 20 July 2010
2010-07-20T01:32:33Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 01:32, 20 July 2010</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 21:</td>
<td colspan="2" class="diff-lineno">Line 21:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>- mOrange E2050* </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>- mOrange E2050* </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>- mCherry J67102*</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>- mCherry J67102*</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br>- mCherry NLS <del class="diffchange diffchange-inline">J130012</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br>- mCherry NLS <ins class="diffchange diffchange-inline">I712028</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>* can have an internal RBS. Check by cloning behind J23100.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>* can have an internal RBS. Check by cloning behind J23100.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>3. Gel electrophoresis:<br>Samples in following order: E240, CFP E0420, YFP E0430, mOrange E2050, mCherry J67102, mCherry NLS J130012</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>3. Gel electrophoresis:<br>Samples in following order: E240, CFP E0420, YFP E0430, mOrange E2050, mCherry J67102, mCherry NLS J130012</div></td></tr>
</table>
Olchowik
http://2010.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Stage1/11_July_2010&diff=25059&oldid=prev
Olchowik: New page: <html> <script type="text/javascript"> ActiveTab='WetLab'; </script> </html> {{TemplateTop}} {{UpperTabs}} {{LowerTabsTeam}} <html> <!-- do not edit above me! --> Monday, 12 June 2010; ...
2010-07-16T20:20:55Z
<p>New page: <html> <script type="text/javascript"> ActiveTab='WetLab'; </script> </html> {{TemplateTop}} {{UpperTabs}} {{LowerTabsTeam}} <html> <!-- do not edit above me! --> Monday, 12 June 2010; ...</p>
<p><b>New page</b></p><div><html><br />
<script type="text/javascript"><br />
ActiveTab='WetLab';<br />
</script><br />
</html><br />
<br />
{{TemplateTop}}<br />
{{UpperTabs}}<br />
{{LowerTabsTeam}}<br />
<html><br />
<!-- do not edit above me! --><br />
<br />
Monday, 12 June 2010;<br />
<br />
<br><br><div class="note">Cloning fluorescent proteins behind expression vector 3.1 and behind promoter J23100</div><br />
1. Digest part part 3.1 – Anderson RBS from distribution (expression vector = promoter J23100+RBS) with SpeI and PstI<br />
<br>2. Digest fluorescent proteins with XbaI and PstI to clone into an expression vector. Fluorescent proteins:<br />
<br>- GFP E240<br />
<br>- CFP E0420<br />
<br>- YFP E0430<br />
<br>- mOrange E2050* <br />
<br>- mCherry J67102*<br />
<br>- mCherry NLS J130012<br />
<br>* can have an internal RBS. Check by cloning behind J23100.<br />
<br>3. Gel electrophoresis:<br>Samples in following order: E240, CFP E0420, YFP E0430, mOrange E2050, mCherry J67102, mCherry NLS J130012<br />
<br>4. Gel extraction and ligation of fluorescent proteins with expression vector.<br />
<div class="note"> GEL </div><br />
Samples: GFP E240, CFP E0420, YFP E0430, mOrange E2050, mCherry J67102, mCherry NLS J130012<br />
<br>Not all samples at expected size, some vectors at wrong size. <br />
<br>Looks like order of some samples is mixed. We will check it **after** transformation, because those are fluorescent proteins therefe it is easy to find the correct samples. Vector with J23100 of expexted size. <br />
<br />
<br><img src="https://static.igem.org/mediawiki/igem.org/8/8e/12_07_FluoProteinsXbaPstandVectorSpePst3_1.JPG"/><br />
<br>______________________________________________<br />
<br>Digest conditions, used for all reactions:<br />
<br>8 μl DNA <br />
<br>2 μl BamHI buffer<br />
<br>10 μl H2O<br />
<br>0,3 μl enzymów <br />
<br>20 μl final volume<br />
<br>digest for 3h<br />
<br>______________________________________________<br />
<br>Ligation conditions, used for all reactions:<br />
<br>25ul insert DNA<br />
<br>2ul vector DNA<br />
<br>3ul ligase buffer<br />
<br>30 ul final volume<br />
<br>overnight ligation<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<!-- do not edit below me! --><br />
</html><br />
{{TemplateBottom}}</div>
Olchowik