Team:Warsaw/Calendar-Stage1/10 September 2010
From 2010.igem.org
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<br> 10.09.2010 Friday | <br> 10.09.2010 Friday | ||
+ | <br>Note: We're not satisfied with the results, because of some problems with the timetable of usage of the cytometer | ||
<br> | <br> | ||
<br><div class="note"> Previous 2 days: Set up and culturing Hela cell culture [Kamil]</div> | <br><div class="note"> Previous 2 days: Set up and culturing Hela cell culture [Kamil]</div> | ||
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- | + | Data from the flow cytometer processed by Cell Quest: | |
+ | <br><br><br/> | ||
<img src="https://static.igem.org/mediawiki/2010/7/71/Bacto.10.09_01.png" width="450px" /> | <img src="https://static.igem.org/mediawiki/2010/7/71/Bacto.10.09_01.png" width="450px" /> | ||
<img src="https://static.igem.org/mediawiki/2010/5/59/Bacto.10.09_02.png" width="800px" /> | <img src="https://static.igem.org/mediawiki/2010/5/59/Bacto.10.09_02.png" width="800px" /> |
Revision as of 16:31, 26 October 2010
10.09.2010 Friday
Note: We're not satisfied with the results, because of some problems with the timetable of usage of the cytometer
Previous 2 days: Set up and culturing Hela cell culture [Kamil]
Use a protocol...
Previous day: Set up liquid cultures of different types of E.coli [Milena]
Use a protocol...
Testing the invasivnes of E.coli in Hela cells [Kamil,Cherry]
1. Incubation of Hela cells with different E.coli types
2. Preparation of probes with incubated cells (use of lysozyme) and examining them in flow cytometer
Data analysis with Cell Quest and Microsoft Excel [Cherry]
legend:
E.coli constructs:
G- GFP
I- Invasin+GFP
J- JPH+Listeriolysin O+Invasin+GFP
A- APH+Listeriolysin O+Invasin+GFP
Hela cells with bacteria:
ML.009- without bacteriaN
ML.010- TOP (50)
ML.003- G (25)
ML.004- I (25)
ML.008- I (50)
ML.011- G (50)
10-09-2010.001- J (50)
10-09-2010.002- A (50)
10-09-2010.003- J (25)
Data from the flow cytometer processed by Cell Quest: