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Team:Warsaw/Calendar-Stage1/10 September 2010
From 2010.igem.org
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| + | <br> 10.09.2010 Friday | ||
| + | <br> | ||
| + | <br><div class="note"> Previous 2 days: Set up and culturing Hela cell culture [Kamil]</div> | ||
| + | <br> Use a protocol... | ||
| + | <br> | ||
| + | <br><div class="note"> Previous day: Set up liquid cultures of different types of E.coli [Milena]</div> | ||
| + | <br> Use a protocol... | ||
| + | <br> | ||
| + | <br><div class="note"> Testing the invasivnes of E.coli in Hela cells [Kamil,Cherry]</div> | ||
| + | <br>1. Incubation of Hela cells with different E.coli types | ||
| + | <br>2. Preparation of probes with incubated cells (use of lysozyme) and examining them in flow cytometer | ||
| + | <br> | ||
| + | <br><div class="note"> Data analysis with Cell Quest and Microsoft Excel [Cherry]</div> | ||
| + | <br> | ||
<br /><strong>legend:</strong> | <br /><strong>legend:</strong> | ||
<hr /> | <hr /> | ||
Revision as of 16:27, 26 October 2010
10.09.2010 Friday
Previous 2 days: Set up and culturing Hela cell culture [Kamil]
Use a protocol...
Previous day: Set up liquid cultures of different types of E.coli [Milena]
Use a protocol...
Testing the invasivnes of E.coli in Hela cells [Kamil,Cherry]
1. Incubation of Hela cells with different E.coli types
2. Preparation of probes with incubated cells (use of lysozyme) and examining them in flow cytometer
Data analysis with Cell Quest and Microsoft Excel [Cherry]
legend:
E.coli constructs:
G- GFP
I- Invasin+GFP
J- JPH+Listeriolysin O+Invasin+GFP
A- APH+Listeriolysin O+Invasin+GFP
Hela cells with bacteria:
ML.009- without bacteriaN
ML.010- TOP (50)
ML.003- G (25)
ML.004- I (25)
ML.008- I (50)
ML.011- G (50)
10-09-2010.001- J (50)
10-09-2010.002- A (50)
10-09-2010.003- J (25)
