Team:Valencia/prion

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Regulating the surface temperature on Mars using a prion switch

Prions

In 1982 Stanley B. Prusiner created the term “prion” (or proteinacius infectious particle) to name the exclusively proteic infectious agent responsible of the transmissible spongiform encephalopathies (TSEs), a group of mammalian neurodegenerative disorders. According with the widely supported “protein-only” model, the prion mechanism of transmissibility arise from the ability of the prion form of the protein to promote the conformational change of the normal cellular form to the infectious prion forms (Prusiner, 1998). The infectious forms are mis-folded proteins that induce by polymerization the formation of an amyloid fold constituted by tightly packed beta sheets. These aggregates are insoluble fibrils that display resistance to proteolytic digestion and have affinity for aromatic dyes.

Fungal prions

In 1994 Reed Wickner proposed the prion nature of Ure2, a protein involved in the nitrogen metabolism of the yeast Saccharomyces cerevisae, to explain the unusual dominant and cytoplasmatic inheritance of the phenotype [URE3] first described by Cox (1965). In later years a wide array of genetic and biochemical evidence have supported that the prionic behaviour is present in other proteins of the yeast such as Sup35, Rnq1 and Swi1 and in HET-s, a protein involved in the mechanism of genetic incompatibility between strains of Podospora anserina.

These prions can produce amyloid-like fibrils similar to those associated with the mammalian prions. The molecular architecture of these amyloids have been studied using solid-state NMR spectroscopy and it has been found that the fibrils formed by Ure2p, Rnq1, and Sup35 share a common parallel and in-register β-structure ().


Sup35p

[PSI+] is a non-mendelian trait of Saccharomyces cerevisae that supress nonsense codons. This phenotype is due to a self-replication conformations of the protein Sup35p, a translation-termination factor. In [psi-] cells, the translation-termination factor Sup35 is soluble and functions with Sup45 to recognize stop codons and terminate translation. In [PSI+] cells, most Sup35 is insoluble and nonfunctional, causing a reduction on translation fidelity. This trait is heritable because Sup35 protein in the [PSI+] conformation influences new Sup35 protein to adopt the same conformation and passes from mother cell to daughter to perpetuate the cycle of conversion. [PSI+] is, however, metaestable: [PSI+] cells occasionally give rise to [psi-] cells and viceversa, as the [PSI+] conformation is lost and gained.

Prion switch

Parts

Behaviour

Yeastworld

Experimental procedure

Results

References