Team:Valencia/Notebook/July

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April
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29 30 31 1  2  3  4
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26 27 28 29 30  1  2
May
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31 1 2 3 4 5 6
June
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28 29 30  1 2 3 4
July
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August
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30 31  1  2  3  4  5
September
Mo Tu We Th Fr Sa Su
30 31  1  2  3  4  5
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27 28 29 30  1  2  3
October
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November
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Contents

July 8th

WETLab meeting

We start to put together all the thing, to begin working in the Lab!! We also made plans for the future.

We prepared the culture medium for growing our yeasts.

Gabi with the super yeast culture medium


Social Event

We got together in Serranos Towers, to chill out a litle bit after the "hard work" in the lab.

Gettin' together in Serranos Tower


July 9th

We filled up the petri dishes with the yeast liquid culture medium. Then we planted the yeasts onto the petri dishes. We prepared an overnight culture at 37ºC into a stove with the bacteria having the plasmids pG1 and pLZ in order to purificate them next Monday.

July 10th

We transferred the bacterial culture from the stove (37ºC) to the fridge (4ºC).


July 11th

Spain won the World cup!!!!


July 12th

We made a miniprep to purify the plasmids pG1 and pLZ.

Gabi Dani reading the protocol and Gabi moral support for the first miniprep!!
Gabi, Jose, JA and Dani's red hair doing the miniprep

Then we carried out a digestion and the following electrophoresis to verify whether we had the constructions in the plasmids and to evaluate their concentration.

We stored the non-digested plasmids in the refrigerator (we want to use it later on) We made a liquid culture of E. coli with the LEA gene, and puted it into the stove at 37ºC. We recieved a filter paper soaked with a plasmid from Kausik Li containing an Aplysia prion fused with the GR domain. Then we put the paper containing the plasmid in water in order to transform and clone it in E. coli later on.

July 13th

We carried out a miniprep to purify the pM2 plasmid containing the LEA gene. Then we carried out a digestion and the following electrophoresis to verify whether we had the constructions in the plasmids and to evaluate their concentration. We performed a transformation of plasmid DNA containing a prionic gene into E.coli following a heat shock protocol.