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Team:Uppsala-SwedenWeek15
From 2010.igem.org
Week-15
Plasmid concentration of K1, K2, K3, (old version) and digestion
|
Sample |
DNA-concentration (µL/ml) |
DNA |
Enzymes (E & P) |
FDB (10X) |
H20 |
Total (µL) |
|
K1C1 |
32,7 |
15.3 |
0,5 &0.5 |
2 |
1.7 |
20 |
|
K1C2 |
98.3 |
5.0 |
0,5 &0.5 |
2 |
12 |
20 |
|
K1C3 |
74.4 |
6.7 |
0,5 &0.5 |
2 |
10.3 |
20 |
|
K1C4 |
516.4 |
0.9 |
0,5 &0.5 |
1 |
7.1 |
10 |
|
K1C5 |
135.4 |
3.6 |
0,5 &0.5 |
1 |
4.4 |
10 |
|
K2C1 |
161.7 |
3.1 |
0,5 &0.5 |
1 |
4.9 |
10 |
|
K2C2 |
92.3 |
5.4 |
0,5 &0.5 |
1 |
2.6 |
10 |
|
K2C3 |
112.2 |
4.3 |
0,5 &0.5 |
1 |
3.7 |
10 |
|
K2C4 |
45.6 |
10.9 |
0,5 &0.5 |
2 |
6.1 |
20 |
|
K2C5 |
348.1 |
1.4 |
0,5 &0.5 |
1 |
6.6 |
10 |
|
K2C6 |
91.3 |
5.4 |
0,5 &0.5 |
1 |
2.6 |
10 |
|
K3C1 |
88.4 |
5.6 |
0,5 &0.5 |
1 |
2.4 |
10 |
|
K3C2 |
109.9 |
4.5 |
0,5 &0.5 |
1 |
3.5 |
10 |
|
K3C3 |
116.6 |
4.3 |
0,5 &0.5 |
1 |
3.8 |
10 |
|
K3C4 |
110.0 |
4.6 |
0,5 &0.5 |
1 |
3.5 |
10 |
|
K3C5 |
90.67 |
5.5 |
0,5 &0.5 |
1 |
2.5 |
10 |
|
K3C6 |
109.5 |
4.6 |
0,5 &0.5 |
1 |
3.4 |
10 |
E= EcorI
P= PstI
FDB= Fast Digest Buffer
Gel of digested samples with 0.8% agarose gel . picture missing
Counting from the ladder. K1(Colony 1-5) and K2(colony 1-6)
