Protocols

Preparing Agar Plates Protocol (Makes 12 (15mm) Plates)

• 300 mL DI H2O + 11 g LB agar
• Autoclave
  • Put control thermometer in H₂O (from the sink)
  • Select vented container mode (Do Not Change Program)
• Mix well after autoclaving; let cool to 50^oC
• Add antibiotic (50 to 100 µg/mL) (15 mg total)
  • Weigh on paper
  • Add to 0.5 mL DI H₂O
  • Add to LB mixture when cool enough
• Plate
  • Under flame open lids of all plates
  • Slowly pour agar into plate, avoiding bubbles, when it touches all edges stop pouring
  • Let sit under flames until gel solidifies
  • Replace lids on plates
• Store upside down at 40^oC