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Team:UTDallas/Protocols
From 2010.igem.org
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=Protocols= | =Protocols= | ||
| + | ===Preparing Agar Plates Protocol (Makes 12 (15mm) Plates)=== | ||
| + | *300 mL DI H2O + 11 g LB agar | ||
| + | *Autoclave | ||
| + | ** Put control thermometer in H<sub>2</sub>O (from the sink) | ||
| + | ** Select vented container mode (Do Not Change Program) | ||
| + | *Mix well after autoclaving; let cool to 50<sup>o</sup>C | ||
| + | *Add antibiotic (50 to 100 µg/mL) (15 mg total) | ||
| + | ** Weigh on paper | ||
| + | ** Add to 0.5 mL DI H<sub>2</sub>O | ||
| + | ** Add to LB mixture when cool enough | ||
| + | *Plate | ||
| + | ** Under flame open lids of all plates | ||
| + | ** Slowly pour agar into plate, avoiding bubbles, when it touches all edges stop pouring | ||
| + | ** Let sit under flames until gel solidifies | ||
| + | ** Replace lids on plates | ||
| + | *Store upside down at 40<sup>o</sup>C | ||
| + | |||
| + | |||
</span> | </span> | ||
Revision as of 16:15, 28 July 2010
Protocols
Preparing Agar Plates Protocol (Makes 12 (15mm) Plates)
- 300 mL DI H2O + 11 g LB agar
- Autoclave
- Put control thermometer in H2O (from the sink)
- Select vented container mode (Do Not Change Program)
- Mix well after autoclaving; let cool to 50oC
- Add antibiotic (50 to 100 µg/mL) (15 mg total)
- Weigh on paper
- Add to 0.5 mL DI H2O
- Add to LB mixture when cool enough
- Plate
- Under flame open lids of all plates
- Slowly pour agar into plate, avoiding bubbles, when it touches all edges stop pouring
- Let sit under flames until gel solidifies
- Replace lids on plates
- Store upside down at 40oC
