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Team:UT-Tokyo/Sudoku data
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(New page: {{UT-Tokyo_HEADER}} <div id="main_all"> = '''Sudoku''' = == '''Protocols and data''' == This is an abstract page. If you can see detail data, please click each protocols. This is an abs...)
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Revision as of 14:10, 23 August 2010
Sudoku
Protocols and data
This is an abstract page. If you can see detail data, please click each protocols. This is an abstract page. If you can see detail data, please click each protocols.
Pre-experiment
234
234
0,i,ii,iii
0,i,ii,iii
Check test
Check test
| Number | Name | Link to BioBrick | Plate coordinate | Vector | Code length |
| 1 | T7 promoter | plate1-6N | pSB1AK8 | 46bp | |
| 2 | rbs | plate1-1H | pSB1A2 | 15bp | |
| 3 | cre recombinase | plate1-5D | pSB1A2 | 1037bp | |
| 4 | double terminator | plate2-24C | pSB1AK3 | 95bp | |
| 5 | lox66 recombinase site | plate1-17J | pSB1A2 | 34bp | |
| 6 | Kan resistance (rev) | plate1-2K | pSB1A2 | 816bp | |
| 7 | rbs (rev) | plate1-1J | pSB1A2 | 15bp | |
| 8 | lox71 recombinase site |
order-made | --- | --- | 34bp |
| 9 | single terminator | plate1-4H | pSB1AK3 | 39bp | |
| 10 | constant express promoter | plate1-18A | pSB1A2 | 35bp | |
| 11 | Tet resistance (rev) | plate1-1N | pSB1A2 | 1191bp |
Main construct
Research of SP6 RNApol cDNA and F plasmid
Research of SP6 RNApol cDNA and F plasmid
Convert lox71(rev) to lox66(rev)
Convert lox71(rev) to lox66(rev)
Make Hin(rev)
Make Hin(rev)
Revise MS2 gene
Revise MS2 gene
Make location(rev)-rbs(rev)-location(rev)
Make location(rev)-rbs(rev)-location(rev)
