Team:UT-Tokyo/Sudoku data

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{{UT-Tokyo_HEADER}}
 
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<div id="main_all">
 
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= '''Sudoku''' =
 
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== '''Data''' ==
 
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== Pre-experiment ==
 
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=== Make construct ===
 
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==== 7/1 ====
 
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'''Transformation'''
 
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2-3 plasmid(6/30 ligation)
 
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<6/30 transformation → fail>
 
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'''Inoculation'''
 
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11 plasmid(6/30 thaw, transformation)
 
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'''Enzyme cut & Gel extract'''
 
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2,3,5,7 plasmid(6/25 miniprep)
 
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2:53.9 ng/uL → about 15 uL(shortage)
 
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3:124.2 ng/uL → 8 uL
 
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5:81.3 ng/uL → 12.3 uL
 
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7:85.4 ng/uL → 11.7 uL
 
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Label: 100701 数X(2,3,5,9) ゲル抽出
 
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=== Check test ===
 
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Check test
 
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{| border="1"
 
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| Number || Name || Link to BioBrick || Plate coordinate || Vector || Code length
 
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|-
 
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| 1 || T7 promoter ||
 
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_I712074  BBa_I712074]
 
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|| plate1-6N || pSB1AK8 || 46bp
 
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|-
 
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| 2 || rbs ||
 
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_B0030  BBa_B0030]
 
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|| plate1-1H || pSB1A2 || 15bp
 
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|-
 
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| 3 || cre recombinase ||
 
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61047  BBa_J61047]
 
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|| plate1-5D || pSB1A2 || 1037bp
 
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|-
 
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| 4 || double terminator ||
 
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_B0014  BBa_B0014]
 
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|| plate2-24C || pSB1AK3 || 95bp
 
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|-
 
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| 5 || lox66 recombinase site ||
 
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_I718017  BBa_I718017]
 
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|| plate1-17J || pSB1A2 || 34bp
 
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|-
 
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| 6 || Kan resistance (rev) ||
 
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_J31002  BBa_J31002]
 
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|| plate1-2K || pSB1A2 || 816bp
 
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| 7 || rbs (rev) ||
 
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_B0014  BBa_B0014]
 
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|| plate1-1J || pSB1A2 || 15bp
 
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|-
 
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| 8 || lox71 recombinase site ||
 
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order-made
 
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|| --- || --- || 34bp
 
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| 9 || single terminator ||
 
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_B1006  BBa_B1006]
 
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|| plate1-4H || pSB1AK3 || 39bp
 
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|-
 
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| 10 || constant express promoter ||
 
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_J23119  BBa_J23119]
 
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|| plate1-18A || pSB1A2 || 35bp
 
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|-
 
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| 11 || Tet resistance (rev) ||
 
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_J31006  BBa_J31006]
 
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|| plate1-1N || pSB1A2 || 1191bp
 
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|}
 
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== Main construct ==
 
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=== Research of SP6 RNApol cDNA and F plasmid ===
 
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Research of SP6 RNApol cDNA and F plasmid
 
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=== Convert lox71(rev) to lox66(rev) ===
 
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Convert lox71(rev) to lox66(rev)
 
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=== Make Hin(rev) ===
 
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Make Hin(rev)
 
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=== Revise MS2 gene ===
 
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Revise MS2 gene
 
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=== Make location(rev)-rbs(rev)-location(rev) ===
 
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Make location(rev)-rbs(rev)-location(rev)
 
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</div>
 
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{{UT-Tokyo_FOOTER}}
 

Latest revision as of 08:29, 27 August 2010