Team:UT-Tokyo/Sudoku data

From 2010.igem.org

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{{UT-Tokyo_HEADER}}
 
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<div id="main_all">
 
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= '''Sudoku''' =
 
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== '''Data''' ==
 
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== Pre-experiment ==
 
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=== Make construct ===
 
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==== 7/1 ====
 
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'''Transformation'''
 
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2-3 plasmid (6/30 ligation)
 
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<6/30 transformation → fail>
 
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'''Inoculation'''
 
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11 plasmid (6/30 thaw, transformation)
 
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'''Enzyme cut'''
 
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2, 3, 5, 7 plasmid (6/25 miniprep)
 
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2 : 53.9 ng/uL → about 15 uL (shortage) / H / SP (2 uL)
 
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3 : 124.2 ng/uL → 8 uL / M / XP (2 uL)
 
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5 : 81.3 ng/uL → 12.3 uL / H / SP (2 uL)
 
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7 : 85.4 ng/uL → 11.7 uL / H / SP (2 uL)
 
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'''Gel extract'''
 
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2, 3, 5, 7 plasmid (7/1 enzyme cut)
 
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Label : 100701 数X (2,3,5,9) ゲル抽出
 
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==== 7/2 ====
 
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'''Miniprep'''
 
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2-3, 11 plasmid (7/1 inoculation)
 
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2-3 → 105 ng/uL
 
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11 (1st) → 191.3 ng/uL
 
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11 (2nd) → 49.5 ng/uL
 
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'''Enzyme cut'''
 
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2-3, 11 plasmid (7/2 miniprep)
 
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2-3 : 9.5 uL / H / ES (1.5 uL)
 
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11 (1st) : 5.2 uL / H /  ES (1.5 uL) → Judged failure by cut check → miniprep again
 
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11 (2nd) : 20.2 uL / H /  ES (1.5 uL)
 
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==== 7/3 ====
 
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'''Gel extract'''
 
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2-3, 11 plasmid (7/2 enzyme cut)
 
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Label : 100703 X ゲル抽出
 
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==== 7/5 ====
 
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'''Ligation'''
 
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2-3 (7/3 enzyme cut) & 4 / 11 (7/3 enzyme cut) & 7
 
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2-3 : 37.5 ng/uL → 5 uL & 4 : 5.2 ng/uL → 5 uL
 
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11 : 4.2 ng/uL → 7.5 uL & 7 : Unknown → 2.5 uL
 
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'''Transformation'''
 
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2-3-4 plasmid (7/5 ligation) / 11-7 plasmid (7/5 ligation)
 
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=== Check test ===
 
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== Main construct ==
 
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=== Research of SP6 RNApol cDNA and F plasmid ===
 
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=== Convert lox71(rev) to lox66(rev) ===
 
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=== Make Hin(rev) ===
 
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=== Revise MS2 gene ===
 
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=== Make location(rev)-rbs(rev)-location(rev) ===
 
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</div>
 
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{{UT-Tokyo_FOOTER}}
 

Latest revision as of 08:29, 27 August 2010