Team:UT-Tokyo/Sudoku data

From 2010.igem.org

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(New page: {{UT-Tokyo_HEADER}} <div id="main_all"> = '''Sudoku''' = == '''Protocols and data''' == This is an abstract page. If you can see detail data, please click each protocols. This is an abs...)
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= '''Sudoku''' =
= '''Sudoku''' =
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== '''Protocols and data''' ==
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== '''Data''' ==
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This is an abstract page.
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If you can see detail data, please click each protocols.
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This is an abstract page.
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If you can see detail data, please click each protocols.
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== Pre-experiment ==
== Pre-experiment ==
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[[Image:Sudoku_const_Pre.png|200px|thumb|Pre-experiment]]
 
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=== 234 ===
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=== Make construct ===
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==== 7/1 ====
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'''Transformation'''
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2-3 plasmid(6/30 ligation)
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<6/30 transformation → fail>
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'''Inoculation'''
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11 plasmid(6/30 thaw, transformation)
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'''Enzyme cut & Gel extract'''
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2,3,5,7 plasmid(6/25 miniprep)
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2:53.9 ng/uL → about 15 uL(shortage)
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3:124.2 ng/uL → 8 uL
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5:81.3 ng/uL → 12.3 uL
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7:85.4 ng/uL → 11.7 uL
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Label: 100701 数X(2,3,5,9) ゲル抽出
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234
 
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=== 0,i,ii,iii ===
 
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0,i,ii,iii
 
=== Check test ===
=== Check test ===

Revision as of 14:34, 23 August 2010

Sudoku

Data

Pre-experiment

Make construct

7/1

Transformation

2-3 plasmid(6/30 ligation)

<6/30 transformation → fail>


Inoculation

11 plasmid(6/30 thaw, transformation)


Enzyme cut & Gel extract

2,3,5,7 plasmid(6/25 miniprep)

2:53.9 ng/uL → about 15 uL(shortage)

3:124.2 ng/uL → 8 uL

5:81.3 ng/uL → 12.3 uL

7:85.4 ng/uL → 11.7 uL

Label: 100701 数X(2,3,5,9) ゲル抽出



Check test

Check test


Number Name Link to BioBrick Plate coordinate Vector Code length
1 T7 promoter

BBa_I712074

plate1-6N pSB1AK8 46bp
2 rbs

BBa_B0030

plate1-1H pSB1A2 15bp
3 cre recombinase

BBa_J61047

plate1-5D pSB1A2 1037bp
4 double terminator

BBa_B0014

plate2-24C pSB1AK3 95bp
5 lox66 recombinase site

BBa_I718017

plate1-17J pSB1A2 34bp
6 Kan resistance (rev)

BBa_J31002

plate1-2K pSB1A2 816bp
7 rbs (rev)

BBa_B0014

plate1-1J pSB1A2 15bp
8 lox71 recombinase site

order-made

--- --- 34bp
9 single terminator

BBa_B1006

plate1-4H pSB1AK3 39bp
10 constant express promoter

BBa_J23119

plate1-18A pSB1A2 35bp
11 Tet resistance (rev)

BBa_J31006

plate1-1N pSB1A2 1191bp


Main construct

Research of SP6 RNApol cDNA and F plasmid

Research of SP6 RNApol cDNA and F plasmid

Convert lox71(rev) to lox66(rev)

Convert lox71(rev) to lox66(rev)

Make Hin(rev)

Make Hin(rev)

Revise MS2 gene

Revise MS2 gene

Make location(rev)-rbs(rev)-location(rev)

Make location(rev)-rbs(rev)-location(rev)


Copyright © 2010 iGEM UT-Tokyo. All rights reserved.