Team:UT-Tokyo/Notebook

From 2010.igem.org

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==Notebook==
==Notebook==
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We started our experiment on July 10th.
Our experiment notebooks (Japanese) can be read [http://www.igem-ut.net/blog/  here (www.igem-ut.net/blog) ].
Our experiment notebooks (Japanese) can be read [http://www.igem-ut.net/blog/  here (www.igem-ut.net/blog) ].
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English Version will come soon!
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== '''Protocol''' ==
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=== Transformation ===
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-preparation
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・iGEM parts / ligation products
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・LBbroth (No antibiotic) 500uL
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・TE 15uL
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・competent cells
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・plates
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・ice box
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・heat block(42℃)
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-Notice
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competent cells
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→ always onice!!! Melt on ice! Mix DNA as soon as cells melt!
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-Protocol
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iGEMパーツを解凍する場合
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↓P200のピペットマンにチップを付け、チップでフィルムに孔を空ける。
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↓add 15µl TE(MilliQで可)、ピペッティング
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↓1µlをコンピテントセルにいれ30min on ice(コンピに対し1/5倍量以下になるように)
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iGEM parts
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Hole film with a tip
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↓Add 15 uL TE or MilliQ, pipetting
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↓Mix 1uL with competent cells 50uL
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↓on ice 30 min
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↓42℃ 45 sec
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↓on ice 10 min
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↓Mix LBbroth 300 uL
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↓37℃ 30 min or room temprature 15 min (amp:omittable)
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↓Plate, 37℃ incubation over night
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Ligation products
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↓Mix ligation products 10uL, competent cells 50uL
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↓on ice 30 min
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↓42℃ 45 sec
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↓on ice 10 min
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↓Mix LBbroth 300 uL
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↓37℃ 30 min or room temprature 15 min (amp:omittable)
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↓Plate, 37℃ incubation over night
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{{UT-Tokyo_FOOTER}}
{{UT-Tokyo_FOOTER}}

Latest revision as of 12:56, 21 August 2010

Notebook

We started our experiment on July 10th.

Our experiment notebooks (Japanese) can be read [http://www.igem-ut.net/blog/ here (www.igem-ut.net/blog) ].

English Version will come soon!

Copyright © 2010 iGEM UT-Tokyo. All rights reserved.