Team:USTC Software/downloads/ADEM/parameter

From 2010.igem.org

(Difference between revisions)
(New page: Parts J23100 through J23119 are a family of constitutive promoters. The absolute promoter strength of J23100 has been measured by [https://2010.igem.org/Team:Warsaw/Stage1/PromMeas|iGEM War...)
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Parts J23100 through J23119 are a family of constitutive promoters. The absolute promoter strength of J23100 has been measured by [https://2010.igem.org/Team:Warsaw/Stage1/PromMeas|iGEM Warsaw 2010 Team] (13,1pg RNA/minute/ug substrate DNA) and the relative promoter strength of other family members are given here [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23100|here]. Our values of ''forwardPromoterEfficiency'' could be easily obtained by unit conversion. For example, it is 2.18E-7 /s. All values of ''forwardPromoterEfficiency'' are listed here to character the activities of those promoters.
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===Characterization of activities of promoters===
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Parts J23100 through J23119 are a family of constitutive promoters. The absolute promoter strength of J23100 has been measured by [https://2010.igem.org/Team:Warsaw/Stage1/PromMeas|iGEM Warsaw 2010 Team] (13,1pg RNA/minute/ug substrate DNA for J23100) and the relative promoter strength of other family members are given here [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23100|here]. Our values of ''forwardPromoterEfficiency'' could be easily obtained by unit conversion. For example, it is 2.18E-7 /s for part J23100. All values of ''forwardPromoterEfficiency'' are listed below to character the activities of those promoters:
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* J23112          1                8.559E-11
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* J23103          17              1.455E-9
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* J23113          21              1.797E-9
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* J23109          106              9.073E-9
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* J23117          162              1.387E-8
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* J23114          256              2.191E-8
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* J23115          387              3.312E-8
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* J23116          396              3.389E-8
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* J23105          623              5.332E-8
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* J23110          844              7.224E-8
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* J23107          908              7.772E-8
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* J23106          1185            1.014E-7
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* J23108          1303            1.115E-7
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* J23118          1429            1.223E-7
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* J23111          1487            1.273E-7
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* J23101          1791            1.533E-7
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* J23104          1831            1.567E-7
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* J23102          2179            1.865E-7
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* J23100          2547            2.180E-7
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where the second column is the relative promoter strength for promoter family members and the third column gives the absolute values.

Revision as of 05:34, 23 October 2010

Characterization of activities of promoters

Parts J23100 through J23119 are a family of constitutive promoters. The absolute promoter strength of J23100 has been measured by Warsaw 2010 Team (13,1pg RNA/minute/ug substrate DNA for J23100) and the relative promoter strength of other family members are given here [1]. Our values of forwardPromoterEfficiency could be easily obtained by unit conversion. For example, it is 2.18E-7 /s for part J23100. All values of forwardPromoterEfficiency are listed below to character the activities of those promoters:

  • J23112 1 8.559E-11
  • J23103 17 1.455E-9
  • J23113 21 1.797E-9
  • J23109 106 9.073E-9
  • J23117 162 1.387E-8
  • J23114 256 2.191E-8
  • J23115 387 3.312E-8
  • J23116 396 3.389E-8
  • J23105 623 5.332E-8
  • J23110 844 7.224E-8
  • J23107 908 7.772E-8
  • J23106 1185 1.014E-7
  • J23108 1303 1.115E-7
  • J23118 1429 1.223E-7
  • J23111 1487 1.273E-7
  • J23101 1791 1.533E-7
  • J23104 1831 1.567E-7
  • J23102 2179 1.865E-7
  • J23100 2547 2.180E-7

where the second column is the relative promoter strength for promoter family members and the third column gives the absolute values.

Retrieved from "http://2010.igem.org/Team:USTC_Software/downloads/ADEM/parameter"