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An Integrated Platform Based on Bacterial Microcompartment for de novo Proteinaceous Artificial Organelles

In the expression of protein, we use and unite the method mentioned in the previous papers. [see protocol] The bacterial was induced by 1 mM IPTG at 30 ℃ for 4 hours. The results was as follows.[the one without pduN was a negative control used to prove the function of pduN: in the growth process, the one without pduN tends to grow with more deposit implying its possible role in the vertex]

SDS-PAGE of pdu proteins

We use the ultracentrifuge to purify the proteins, with 48000 g speed, we get our targeted proteins in the pellet.

SDS-PAGE of pdu proteins

Finally, we got the E.coli with the Pdu microcompartment tested in TEM

We are excited to see the result we expected, in this TEM result we can see the Pdu microcompartment were successfully assemblied in E.coli.

Experimental group:E.coli with BMC