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An Integrated Platform Based on Bacterial Microcompartment for de novo Proteinaceous Artificial Organelles

In the expression of protein, we use and unite the method mentioned in the previous papers. [see protocol] The bacterial was induced by 1 mM IPTG at 30 ℃ for 4 hours. The results was as follows.[the one without pduN was a negative control used to prove the function of pduN: in the growth process, the one without pduN tends to grow with more deposit implying its possible role in the vertex] SDS-PAGE of pdu proteins

We use the ultracentrifuge to purify the proteins, with 48000 g speed, we get our targeted proteins in the pellet. SDS-PAGE of pdu proteins