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Team:USTC/Notebook/Meetings/4 September 2010
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Evelynzhang (Talk | contribs) (New page: Evelyn: Transformation of the ligation product: pduJK-NU and pduNT-UV.) |
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Transformation of the ligation product: pduJK-NU and pduNT-UV. | Transformation of the ligation product: pduJK-NU and pduNT-UV. | ||
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| + | Hilde: | ||
| + | |||
| + | select single colony from plate of transformation product of pduABJKNTUV | ||
| + | |||
| + | Colony PCR test for pduABJKNTUV | ||
| + | |||
| + | Fastdigest cutting: Promotor(Insert, E,S) & pduABJKNTUV(Vector, E,X) | ||
| + | |||
| + | Miniprep: plasmids of pduABJKNTUV | ||
| + | |||
| + | DNA Ligation Kit LONG: Prmotor+pduABJKNTUV (overnight) | ||
Latest revision as of 07:31, 22 September 2010
Evelyn:
Transformation of the ligation product: pduJK-NU and pduNT-UV.
Hilde:
select single colony from plate of transformation product of pduABJKNTUV
Colony PCR test for pduABJKNTUV
Fastdigest cutting: Promotor(Insert, E,S) & pduABJKNTUV(Vector, E,X)
Miniprep: plasmids of pduABJKNTUV
DNA Ligation Kit LONG: Prmotor+pduABJKNTUV (overnight)
