Team:USTC/Notebook/Meetings/1 September 2010

From 2010.igem.org

Jocelyn:

miniprep of pdu J(PSB1C7)

miniprep of pduK(PSB1C7)


Zocean:

transformation :

[pduA+midRBS+pduB]


LiuChang:

construction of plasmids of the new standard:

ligation of pduN+pduT

ligation of pduU+pduV

ligation of pduJ +pduK


Evelyn:

Restriction Enzyme Cutting of pduN-U and pduN-V.


Hilde:

miniprep of pduNTUV

Fastdigest cutting of pduNTUV(Insert,E,S)

Fastdigest cutting of pduABJK(Vector,E,X)

DNA Ligation Kit LONG of pduABJKNTUV (overnight)

SolutionI Ligation of pduABJKNTUV (overnight)