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Team:USTC/Notebook/Meetings/1 September 2010
From 2010.igem.org
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Restriction Enzyme Cutting of pduN-U and pduN-V. | Restriction Enzyme Cutting of pduN-U and pduN-V. | ||
| + | |||
| + | ---- | ||
| + | |||
| + | Hilde: | ||
| + | |||
| + | miniprep of pduNTUV | ||
| + | |||
| + | Fastdigest cutting of pduNTUV(Insert,E,S) | ||
| + | |||
| + | Fastdigest cutting of pduABJK(Vector,E,X) | ||
| + | |||
| + | DNA Ligation Kit LONG of pduABJKNTUV (overnight) | ||
| + | |||
| + | SolutionI Ligation of pduABJKNTUV (overnight) | ||
Latest revision as of 06:37, 22 September 2010
Jocelyn:
miniprep of pdu J(PSB1C7)
miniprep of pduK(PSB1C7)
Zocean:
transformation :
[pduA+midRBS+pduB]
LiuChang:
construction of plasmids of the new standard:
ligation of pduN+pduT
ligation of pduU+pduV
ligation of pduJ +pduK
Evelyn:
Restriction Enzyme Cutting of pduN-U and pduN-V.
Hilde:
miniprep of pduNTUV
Fastdigest cutting of pduNTUV(Insert,E,S)
Fastdigest cutting of pduABJK(Vector,E,X)
DNA Ligation Kit LONG of pduABJKNTUV (overnight)
SolutionI Ligation of pduABJKNTUV (overnight)
