"

From 2010.igem.org

Evelyn:

PCR: pduN in new standard.

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daodao

colony PCR: pduN in new standard

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Knightmare:

Plasmid extraction of pduN & pduJK on PSB1C3 in old standard.

Digestion: pduJK & pduN/pduTUV on PSB1C3.