Team:USTC/Notebook/Meetings/17 August 2010

From 2010.igem.org

Evelyn:

Plasmid PCR: PSB1C3 with the primer 1C3_f1 and 1C3_r2.

Plasmid PCR: PSB1C3 with the mutation.

Enzyme digestion of PSB1C3_f3r4.


Knightmare:

Plasmid extraction of pduTUV on PSB1C3 in old standard and send for squencing.

Plasmid extraction of pduAB & pduTUV on PSB1C3 in old standard.

Plasmid extraction of pduJK on PSB1C3 in old standard and sendd for squencing.

Amplify from single clone: pduN.