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From 2010.igem.org

Jocelyn:

Double Digest of pduA, pduB in BBF RFC 53 manner. => failed in A...T.T => Digest pduA again.

Ligation of pduJK (solutino I & solution I+II)、pduNU、plasimd with RBS.

Add 1mM IPTG in bacteria in LB to induce protein production in 16℃、30℃.

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Hilde:

Fastdigest cutting: pduABJKNTUV(E,X)

Ligation of Promotor-pduABJKNTUV

Transformation of ligation produce

Coating of Promotor-pduABJKNTUV onto Cl+ plate