Team:USTC/Notebook/Meetings/15 September 2010
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Revision as of 14:20, 15 September 2010 by Jocelynatustc (Talk | contribs)
Jocelyn:
Double Digest of pduA, pduB in BBF RFC 53 manner. => failed in A...T.T => Digest pduA again.
Ligation of pduJK (solutino I & solution I+II)、pduNU、plasimd with RBS.
Add 1mM IPTG in bacteria in LB to induce protein production in 16℃、30℃.