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From 2010.igem.org

Revision as of 09:40, 22 September 2010 (view source) Hildegard (Talk | contribs) ← Older edit		Latest revision as of 09:43, 22 September 2010 (view source) Hildegard (Talk | contribs)
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	Hilde:		Hilde:
-	PCR test of pduN (N-r, N-f) Model: NTUV plm.-1/-2/-3; NTUV plm.2 No.1-2-3-4; ABJKNTUV#2-#9-#5;TUV5(AD);NULL	+	Fastdigest cutting: pduABJKNTUV(E,X)
		+
		+	Ligation of Promotor-pduABJKNTUV
		+
		+	Transformation of ligation produce
		+
		+	Coating of Promotor-pduABJKNTUV onto Cl+ plate

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Latest revision as of 09:43, 22 September 2010

Jocelyn:

Double Digest of pduA, pduB in BBF RFC 53 manner. => failed in A...T.T => Digest pduA again.

Ligation of pduJK (solutino I & solution I+II)、pduNU、plasimd with RBS.

Add 1mM IPTG in bacteria in LB to induce protein production in 16℃、30℃.

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Hilde:

Fastdigest cutting: pduABJKNTUV(E,X)

Ligation of Promotor-pduABJKNTUV

Transformation of ligation produce

Coating of Promotor-pduABJKNTUV onto Cl+ plate