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Team:USTC/Notebook/Meetings/15 September 2010
From 2010.igem.org
(Difference between revisions)
(New page: Jocelyn: Double Digest of pduA, pduB in RFC 53 manner. => failed in A...T.T => Digest pduA again. Ligation of pduJK (solutino I & solution I+II)、pduNU、plasimd with RBS. Add 1mM IPTG...) |
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Jocelyn: | Jocelyn: | ||
| - | Double Digest of pduA, pduB in RFC 53 manner. => failed in A...T.T => Digest pduA again. | + | Double Digest of pduA, pduB in BBF RFC 53 manner. => failed in A...T.T => Digest pduA again. |
Ligation of pduJK (solutino I & solution I+II)、pduNU、plasimd with RBS. | Ligation of pduJK (solutino I & solution I+II)、pduNU、plasimd with RBS. | ||
Add 1mM IPTG in bacteria in LB to induce protein production in 16℃、30℃. | Add 1mM IPTG in bacteria in LB to induce protein production in 16℃、30℃. | ||
| + | |||
| + | ---- | ||
| + | Hilde: | ||
| + | |||
| + | Fastdigest cutting: pduABJKNTUV(E,X) | ||
| + | |||
| + | Ligation of Promotor-pduABJKNTUV | ||
| + | |||
| + | Transformation of ligation produce | ||
| + | |||
| + | Coating of Promotor-pduABJKNTUV onto Cl+ plate | ||
Latest revision as of 09:43, 22 September 2010
Jocelyn:
Double Digest of pduA, pduB in BBF RFC 53 manner. => failed in A...T.T => Digest pduA again.
Ligation of pduJK (solutino I & solution I+II)、pduNU、plasimd with RBS.
Add 1mM IPTG in bacteria in LB to induce protein production in 16℃、30℃.
Hilde:
Fastdigest cutting: pduABJKNTUV(E,X)
Ligation of Promotor-pduABJKNTUV
Transformation of ligation produce
Coating of Promotor-pduABJKNTUV onto Cl+ plate
