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Team:USTC/Notebook/Meetings/14 September 2010
From 2010.igem.org
(Difference between revisions)
(New page: Jocelyn: Pick and amplify the single colony from the promoter-pdu LB plate. 1 each (prep for protein expression) Miniprep of the promoter-pdu. 5 in all PduAB construction failed. Forgot...) |
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PduAB construction failed. Forgot to add the mid-part. T.T => Double Digest plasmid again. | PduAB construction failed. Forgot to add the mid-part. T.T => Double Digest plasmid again. | ||
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| + | ---- | ||
| + | Hilde: | ||
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| + | Fastdigest text: plm. of GFP & RFP | ||
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| + | Colony PCR test (VR2,ABr3) | ||
| + | |||
| + | Fastdigest cutting: Promotor(Insert,E,S) & ABJKNTUV(Vector,E,X) | ||
Latest revision as of 09:34, 22 September 2010
Jocelyn:
Pick and amplify the single colony from the promoter-pdu LB plate. 1 each (prep for protein expression)
Miniprep of the promoter-pdu. 5 in all
PduAB construction failed. Forgot to add the mid-part. T.T => Double Digest plasmid again.
Hilde:
Fastdigest text: plm. of GFP & RFP
Colony PCR test (VR2,ABr3)
Fastdigest cutting: Promotor(Insert,E,S) & ABJKNTUV(Vector,E,X)
