Team:USTC/Notebook

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== Preliminary work ==
== Preliminary work ==

Revision as of 20:23, 27 October 2010

An Integrated Platform Based on Bacterial Microcompartment for de novo Proteinaceous Artificial Organelles

Preliminary work

  • From Feb.4th to Feb.13th, a collective training is held targeting to the practice of basic experiment. Setting GFP protein expression system as an example, we learn from the program how to construct standard components and then an expression system. It`s the time we get to know each other and become a team as a whole.
    • The contents of the training are listed as followed:
      • 1. outlines
      • 2. E.coli culture (Medium preparation, Inoculation, Aseptic operation)
      • 3. plasmid extraction
      • 4. PCR
      • 5. DNA Electrophoresis
      • 6. Double Digest
      • 7. reclaim and appraise of the product of enzyme cutting
      • 8. Ligation
      • 9. preparation of competent cell
      • 10. genomic screening of masculine clone
      • 11. DNA sequencing

Lab Notebook

Meetings are set every Saturday since Feb.15th, then three more discussions (Mon, Wes, Fri) are added when we start to determine our project since Mar 21th.

  • 2010.4.10 – 2010.5.14 reports of the former iGEM works
  • 2010.5.21 – 2010.6.11 broach of candidate projects
    • cell density control
    • Reporter and imaging
    • DNA writing and reading in the cell
    • Biodegradation of plastics
  • 2010.6.12 – 2010.6.30 further research by small groups on the candidate projects
  • sketch map of our final project host-cell free reaction chamber was set up

All the reports information, slides and audio records of our meetings are arranged here.

June
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
July
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
August
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31
September
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
October
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
November
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30