Team:USTC/Modeling/a

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[5] Structural analysis of CsoS1A and the protein shell of the Halothiobacillus neapolitanus carboxysome. ''PLoS Biol.'' 2007 Jun;'''5'''(6):e144.
[5] Structural analysis of CsoS1A and the protein shell of the Halothiobacillus neapolitanus carboxysome. ''PLoS Biol.'' 2007 Jun;'''5'''(6):e144.
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[6] Protein content of polyhedral organelles involved in coenzyme B12-dependent degradation of 1,2-propanediol in Salmonella enterica serovar Typhimurium LT2. ''J Bacteriol.'' 2003 Sep;'''185'''(17):5086-95

Revision as of 01:42, 25 October 2010

An Integrated Platform Based on Bacterial Microcompartment for de novo Proteinaceous Artificial Organelles

Known Conditions

This table illustrate some basic information of pdu shell protein family.[1]


USTC2010_known_conditions_table_1.jpg

Table 1| Basic information of pdu shell protein family.


USTC2010_known_conditions_figure_1.jpg

Figure 1| 3-dimension structure of the known shell proteins from Salmonella enterica . UPPER: pduU. [2] MIDDLE: pduA. [3] DOWN:pduT. [3]

Hypothesis

I)Pdu N is the vertex and its pentametic structuresfor its identity with a proved vertex pentametic protein---CcmL. [4]

II) Hexamers forms the facets, which comprises the building blocks of bacterial microcompartment shells. [5]

USTC2010_hypothesis_N_vertex.jpg USTC2010_Hypothesis_Hexamer.jpg

Figure 2| LEFT: A diagram showing the construction of a large icosahedron from many smaller hexagons and 12 pentagons at the vertices. The figure shown has a triangulation number (T) of 75 (29). RIGHT: Individual CsoS1A molecules, typical shell proteins, are differently in each hexamer.

III) Similarity of the shell proteins to the carboxysome [6] and carboxysome is believed to be in icosohedral shape.

Results

Conclusion

Reference

[1] Martin J. Warren, et al. 2010. Synthesis of empty bacterial microcompartments, directed organelle protein incorporation, and evidence of filament-associated organelle movement. Molecular Cell. 2010 Apr 38 (2), 305–315

[2] Structure of the PduU shell protein from the Pdu microcompartment of Salmonella. Structure. 2008 Sep 16(9):1324-32

[3] Structural insights into the mechanisms of transport across the Salmonella enterica Pdu microcompartment shell. J Biol Chem. 2010 Sep: [Epub ahead of print]

[4] Atomic-level models of the bacterial carboxysome shell. Science. 2008 Feb, 319(5866):1083-6

[5] Structural analysis of CsoS1A and the protein shell of the Halothiobacillus neapolitanus carboxysome. PLoS Biol. 2007 Jun;5(6):e144.

[6] Protein content of polyhedral organelles involved in coenzyme B12-dependent degradation of 1,2-propanediol in Salmonella enterica serovar Typhimurium LT2. J Bacteriol. 2003 Sep;185(17):5086-95