Team:UPO-Sevilla/Project

From 2010.igem.org

(Difference between revisions)
 
(20 intermediate revisions not shown)
Line 1: Line 1:
 +
<div class=globalBC>
{{:Team:UPO-Sevilla/header}}
{{:Team:UPO-Sevilla/header}}
<!--  -->
<!--  -->
Line 4: Line 5:
         <script type="text/javascript" language="javascript">
         <script type="text/javascript" language="javascript">
         <!--
         <!--
-
             current("project","https://static.igem.org/mediawiki/2010/6/6e/BannerHomeBacterialCrowding.jpg");
+
             current("project","https://static.igem.org/mediawiki/2010/3/3b/BannerProjectBacterialCrowding.png");
         -->
         -->
         </script>
         </script>
</html>
</html>
 +
<html>
<div class=contentBC>
<div class=contentBC>
-
<br /><br />
+
<h1>Abstract</h1>
-
<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert ***
+
<p>The aim of our project is to concentrate a significant population of bacteria around a vegetable polysaccharidic surface, a <strong>non-difussible signal</strong>. This phenomenon was renamed as Bacterial Crowding. Therefore, it is necessary that the process of interaction with the surface by a few number of bacteria triggered the production and excretion of chemicals that, acting as <strong>chemoattractants</strong>, generate a chemical-diffusing gradient which could induce chemotactic process of close bacteria. These bacteria will redirect their random movements to the same plant surface and the concentration of the cell population will raise up in this region, thanks to an amplification process.</p>
-
<html>
+
<p>In order to get that effect it is necessary to use the <strong>Prh system</strong>, the only known sensing system able to detect non-diffusble signals. We also used the Fec system to design four signal transduction circuits which would finish with PrhI-dependent promoter PprhJ, which would activate specifically chemoattractant production (aspartate, glutamate or salicylate); or with FecI-dependent promoter PfecA, which would act in the same way. You can see these circuits <a href="https://2010.igem.org/Team:UPO-Sevilla/Biobricks/Circuits" target="_blank">here</a>. However, we finally only focused on one signal transduction circuit which uses PrhA/FecA outer membrane fusion protein, performing an <strong>hybrid sensing system</strong>.</p>
-
<div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;">
+
-
<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
+
-
This is a template page. READ THESE INSTRUCTIONS.
+
-
</div>
+
-
<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
+
-
You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
+
-
</div>
+
-
<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
+
-
You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page. PLEASE keep all of your pages within your teams namespace. 
+
-
</div>
+
-
</div>
+
-
</html>
+
-
*** End of the alert box *** -->
+
<p>This project could use one or two bacterial strains, depending on the chemoattractant. If it is aspartate or glutamate it would be necessary only an <i>E. coli</i> population that uses as chemotactic receptor Tar. If we use salicylate as chemoatracttant, the system will be composed by two bacterial strains: <i>Escherichia coli</i> (detection and signaling population) and <i>Pseudomonas putida G7</i> (chemotactic population), because the first one is not chemotactic to salicylate.
 +
</p>
-
{|align="justify"
+
<div class="center">
-
|You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
+
                  <a href="https://2010.igem.org/Team:UPO-Sevilla/Project/Sensing"><img class="subBanner" src="https://static.igem.org/mediawiki/2010/f/f8/BacterialCrowdingSensingBanner.png" alt="Sensing" /></a>
-
|[[Image:UPO-Sevilla_logo.png|200px|right|frame]]
+
                  <a href="https://2010.igem.org/Team:UPO-Sevilla/Project/Chemotaxis"> <img class="subBanner" src="https://static.igem.org/mediawiki/2010/1/1f/BacterialCrowdingChemotaxisBanner.png" alt="Chemotaxis" /></a>
-
|-
+
</div>
-
|
+
<div class="center">
-
''Tell us more about your project.  Give us background. Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs)''
+
                  <a href="https://2010.igem.org/Team:UPO-Sevilla/Project/Assays"><img class="subBanner" src="https://static.igem.org/mediawiki/2010/0/0d/BacterialCrowdingAssaysBanner.png" alt="Assays" /></a>
-
|[[Image:Sunflowers_pic_small.jpg|right|frame|Your team picture]]
+
                  <a href="https://2010.igem.org/Team:UPO-Sevilla/Project/Results"><img class="subBanner" src="https://static.igem.org/mediawiki/2010/d/dd/BacterialCrowdingResultsBanner.png" alt="Results" /></a>
-
|-
+
</div>
-
|
+
-
|align="center"|[[Team:UPO-Sevilla | Team Example]]
+
-
|}
+
-
== '''Overall project''' ==
 
-
 
-
Your abstract
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
== Project Details==
 
-
 
-
 
-
 
-
 
-
 
-
=== Part 2 ===
 
-
 
-
 
-
 
-
 
-
 
-
=== The Experiments ===
 
-
 
-
 
-
 
-
 
-
=== Part 3 ===
 
-
 
-
 
-
 
-
 
-
== Results ==
 
</div>
</div>
 +
</html>
{{:Team:UPO-Sevilla/footer}}
{{:Team:UPO-Sevilla/footer}}
 +
</div>

Latest revision as of 18:47, 27 October 2010

Abstract

The aim of our project is to concentrate a significant population of bacteria around a vegetable polysaccharidic surface, a non-difussible signal. This phenomenon was renamed as Bacterial Crowding. Therefore, it is necessary that the process of interaction with the surface by a few number of bacteria triggered the production and excretion of chemicals that, acting as chemoattractants, generate a chemical-diffusing gradient which could induce chemotactic process of close bacteria. These bacteria will redirect their random movements to the same plant surface and the concentration of the cell population will raise up in this region, thanks to an amplification process.

In order to get that effect it is necessary to use the Prh system, the only known sensing system able to detect non-diffusble signals. We also used the Fec system to design four signal transduction circuits which would finish with PrhI-dependent promoter PprhJ, which would activate specifically chemoattractant production (aspartate, glutamate or salicylate); or with FecI-dependent promoter PfecA, which would act in the same way. You can see these circuits here. However, we finally only focused on one signal transduction circuit which uses PrhA/FecA outer membrane fusion protein, performing an hybrid sensing system.

This project could use one or two bacterial strains, depending on the chemoattractant. If it is aspartate or glutamate it would be necessary only an E. coli population that uses as chemotactic receptor Tar. If we use salicylate as chemoatracttant, the system will be composed by two bacterial strains: Escherichia coli (detection and signaling population) and Pseudomonas putida G7 (chemotactic population), because the first one is not chemotactic to salicylate.

Assays Results
Footer