Team:UPO-Sevilla/Notebook/09 29

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       <h2>Assay Team</h2>
       <h2>Assay Team</h2>
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       <p>We analyzed plates spread in the previus chemotaxis assay. There were not colonies in plates coming from non succinate cubicles. That could meant that <i>P. putida</i> KT2442 needs succinate as energy source to show chemotaxis behavior. We wanted to study if the results were reproducible and in this case they were not. It was supposed that needles with the same content and in the same cubicle should have shown a similar number of colonies in the results. But only in the 10mM Asp + Succ cubicle that was
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       <p>We have analyzed plates spread in the previous chemotaxis assay. There were no colonies coming from non succinate cubicles in those plates. That could mean that <i>P. Putida</i> KT2442 needs succinate as an energy source to show chemotaxis behaviour. We wanted to observe if the results were reproducible and in this case they were not.Needles are supposed to show a similar number of colonies when having the same content and being in the same cubicle. Yet only in the 10mM Asp + Succ cubicle that would
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truth. We had to continue working on the design of the chemotaxis assays to achieve reproducible results. Finally we could observe a tendency to increase the number of bacteria in needles with aspartate (in cubicles with succinate). Nonetheless, owing to the
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happen. We had to continue working on the design of the chemotaxis assays to achieve reproducible results. Finally we could observe that there was a tendency to increase the number of bacteria in needles when using aspartate (in cubicles with succinate). Nonetheless, owing to the
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non reproducible results, we could not admit that like a success.</p>
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non reproducible results, we could not admit that as a success.</p>
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Revision as of 23:14, 19 October 2010

September, 29th

Assay Team

We have analyzed plates spread in the previous chemotaxis assay. There were no colonies coming from non succinate cubicles in those plates. That could mean that P. Putida KT2442 needs succinate as an energy source to show chemotaxis behaviour. We wanted to observe if the results were reproducible and in this case they were not.Needles are supposed to show a similar number of colonies when having the same content and being in the same cubicle. Yet only in the 10mM Asp + Succ cubicle that would happen. We had to continue working on the design of the chemotaxis assays to achieve reproducible results. Finally we could observe that there was a tendency to increase the number of bacteria in needles when using aspartate (in cubicles with succinate). Nonetheless, owing to the non reproducible results, we could not admit that as a success.

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