Team:UPO-Sevilla/Notebook/09 20

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September, 20th

Assay Team

We designed and prepared material to performance an assay to show Pseudomonas sp. G7 was chemotactic to salicilate. We tested two kind of needles (more or less thin) and three different concentrations of salicilate: 1mM, 10mM and 100mM. In each chemotaxis cubicle there were two needles: one with salicilate (S) and another with buffer, the control (C). This is a representation of the assay:

  1mM 10mM 100mM
thin needle
S        C
S        C
S        C
thick needle
S        C
S        C
S        C
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