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Team:UPO-Sevilla/Notebook/09 14
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<p>Minipreps of (1+2) and (16+3) to get the plasmids. Digestion of the first one with the restriction enzymes SpeI and PstI, and the second one with XbaI and PstI, that is to say, using standard assembly We run an electrophoresis to isolate and purify the suitable fragments. We ligate them o/n.</p> | <p>Minipreps of (1+2) and (16+3) to get the plasmids. Digestion of the first one with the restriction enzymes SpeI and PstI, and the second one with XbaI and PstI, that is to say, using standard assembly We run an electrophoresis to isolate and purify the suitable fragments. We ligate them o/n.</p> | ||
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| + | <h2>Assay Team</h2> | ||
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| + | <p>We develop different assays by changing different features; the plates are incubated overnight.</p> | ||
<a class="return_button" href="/Team:UPO-Sevilla/Notebook" title="Notebook"><span>Return to Notebook</span></a> | <a class="return_button" href="/Team:UPO-Sevilla/Notebook" title="Notebook"><span>Return to Notebook</span></a> | ||
Revision as of 17:04, 17 October 2010
September, 14th
Assembly Team
Minipreps of (1+2) and (16+3) to get the plasmids. Digestion of the first one with the restriction enzymes SpeI and PstI, and the second one with XbaI and PstI, that is to say, using standard assembly We run an electrophoresis to isolate and purify the suitable fragments. We ligate them o/n.
Assay Team
We develop different assays by changing different features; the plates are incubated overnight.
Return to Notebook
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