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Team:UPO-Sevilla/Notebook/09 10
From 2010.igem.org
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<p>We realized a new ligation of the failed biobricks and digested not available biobricks (2+6) and vectors (pSB1C3 and pSB1T3).</p> | <p>We realized a new ligation of the failed biobricks and digested not available biobricks (2+6) and vectors (pSB1C3 and pSB1T3).</p> | ||
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| + | <h2>Assay Team</h2> | ||
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| + | <p>We develop different assays by changing different features; the plates are incubated overnight.</p> | ||
<a class="return_button" href="/Team:UPO-Sevilla/Notebook" title="Notebook"><span>Return to Notebook</span></a> | <a class="return_button" href="/Team:UPO-Sevilla/Notebook" title="Notebook"><span>Return to Notebook</span></a> | ||
Revision as of 17:01, 17 October 2010
September, 10th
Production Team
David Caballero. We change some conditions to improve our PCR results:
- We used new Pfu enzyme, buffer and dNTP.
- Templates: we diluted the high concentrated fragment (1/10) and used 2ul instead of 1ul of the low concentrated one.
0’8% gel electrophoresis analysis showed a line of 1Kb, which is the length of fecR. There was also a 0’2Kb line. It looked like that the new conditions carried us to the goal. We had to isolate from gel the new part.
Assembly Team
We realized a new ligation of the failed biobricks and digested not available biobricks (2+6) and vectors (pSB1C3 and pSB1T3).
Assay Team
We develop different assays by changing different features; the plates are incubated overnight.
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Sponsors
