http://2010.igem.org/wiki/index.php?title=Team:UPO-Sevilla/Notebook/09_08&feed=atom&action=historyTeam:UPO-Sevilla/Notebook/09 08 - Revision history2024-03-29T14:46:35ZRevision history for this page on the wikiMediaWiki 1.16.5http://2010.igem.org/wiki/index.php?title=Team:UPO-Sevilla/Notebook/09_08&diff=151530&oldid=prevAdrian at 23:51, 25 October 20102010-10-25T23:51:23Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p<del class="diffchange diffchange-inline">><strong>David Caballero.</strong</del>> We performed the second overlapping PCR reaction of the SDM with the former and purified PCR products. We carried out two strategies: in one PCR reaction we used the original concentration and in another we used a 1/10 dilution of it.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p> We performed the second overlapping PCR reaction of the SDM with the former and purified PCR products. We carried out two strategies: in one PCR reaction we used the original concentration and in another we used a 1/10 dilution of it.</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p>Products of these PCR reactions were analyzed in 0,8% gel electrophoresis and it was shown that there were not amplification.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p>Products of these PCR reactions were analyzed in 0,8% gel electrophoresis and it was shown that there were not amplification.</p></div></td></tr>
</table>Adrianhttp://2010.igem.org/wiki/index.php?title=Team:UPO-Sevilla/Notebook/09_08&diff=131521&oldid=prevLepavgom at 12:22, 24 October 20102010-10-24T12:22:41Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <h1>September, <del class="diffchange diffchange-inline">08th</del></h1></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <h1>September, <ins class="diffchange diffchange-inline">8th</ins></h1></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p>We carried out the assay without any problem but we must wait to see the results. We have to incubate the plates overnight.</p> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p>We carried out the assay without any problem but we must wait to see the results. We have to incubate the plates overnight.</p> </div></td></tr>
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</table>Lepavgomhttp://2010.igem.org/wiki/index.php?title=Team:UPO-Sevilla/Notebook/09_08&diff=106737&oldid=prevLepavgom at 16:59, 17 October 20102010-10-17T16:59:46Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p>Transformation of <i>E. coli</i> DH5-alfa with the products of ligations, and we spread in selective plates.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p>Transformation of <i>E. coli</i> DH5-alfa with the products of ligations, and we spread in selective plates.</p></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <h2>Assay Team</h2></ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <p>We carried out the assay without any problem but we must wait to see the results. We have to incubate the plates overnight.</p> </ins></div></td></tr>
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</table>Lepavgomhttp://2010.igem.org/wiki/index.php?title=Team:UPO-Sevilla/Notebook/09_08&diff=106654&oldid=prevLepavgom: New page: <div class=globalBC> {{:Team:UPO-Sevilla/header}} <!-- --> <html> <head> <script type="text/javascript" language="javascript"> <!-- current("notebook","http...2010-10-17T16:40:22Z<p>New page: <div class=globalBC> {{:Team:UPO-Sevilla/header}} <!-- --> <html> <head> <script type="text/javascript" language="javascript"> <!-- current("notebook","http...</p>
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<h1>September, 08th</h1><br />
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<h2>Production Team</h2><br />
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<p><strong>David Caballero.</strong> We performed the second overlapping PCR reaction of the SDM with the former and purified PCR products. We carried out two strategies: in one PCR reaction we used the original concentration and in another we used a 1/10 dilution of it.</p><br />
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<p>Products of these PCR reactions were analyzed in 0,8% gel electrophoresis and it was shown that there were not amplification.</p><br />
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<h2>Assembly Team</h2><br />
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<p>Transformation of <i>E. coli</i> DH5-alfa with the products of ligations, and we spread in selective plates.</p><br />
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<a class="return_button" href="/Team:UPO-Sevilla/Notebook" title="Notebook"><span>Return to Notebook</span></a><br />
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