Team:UPO-Sevilla/Notebook/09 08

From 2010.igem.org

(Difference between revisions)
Line 15: Line 15:
<html>
<html>
-
       <h1>September, 08th</h1>
+
       <h1>September, 8th</h1>
       <h2>Production Team</h2>
       <h2>Production Team</h2>
Line 30: Line 30:
       <p>We carried out the assay without any problem but we must wait to see the results. We have to incubate the plates overnight.</p>   
       <p>We carried out the assay without any problem but we must wait to see the results. We have to incubate the plates overnight.</p>   
 +
 +
    <a class="return_button" href="/Team:UPO-Sevilla/Notebook/09_07" title="Go to 7th of September"><span>Go to 7th of September</span></a>
 +
 +
    <a class="next_button" href="/Team:UPO-Sevilla/Notebook/09_09" title="Go to 9th of September"><span>Go to 9th of September</span></a>
 +
 +
    <div class="clear"></div>
     <a class="return_button" href="/Team:UPO-Sevilla/Notebook" title="Notebook"><span>Return to Notebook</span></a>
     <a class="return_button" href="/Team:UPO-Sevilla/Notebook" title="Notebook"><span>Return to Notebook</span></a>

Revision as of 12:22, 24 October 2010

September, 8th

Production Team

David Caballero. We performed the second overlapping PCR reaction of the SDM with the former and purified PCR products. We carried out two strategies: in one PCR reaction we used the original concentration and in another we used a 1/10 dilution of it.

Products of these PCR reactions were analyzed in 0,8% gel electrophoresis and it was shown that there were not amplification.

Assembly Team

Transformation of E. coli DH5-alfa with the products of ligations, and we spread in selective plates.

Assay Team

We carried out the assay without any problem but we must wait to see the results. We have to incubate the plates overnight.

Go to 7th of September Go to 9th of September
Return to Notebook

Footer