Team:UPO-Sevilla/Notebook/09 01

From 2010.igem.org

(Difference between revisions)
(New page: <div class=globalBC> {{:Team:UPO-Sevilla/header}} <!-- --> <html> <head> <script type="text/javascript" language="javascript"> <!-- current("notebook","http...)
Line 40: Line 40:
       <p>We wrote protocols for next devices, SDM of UPO28 and digestion analysis of 1+19 and 16+3.</p>
       <p>We wrote protocols for next devices, SDM of UPO28 and digestion analysis of 1+19 and 16+3.</p>
-
      
+
 
 +
     <a class="return_button" href="/Team:UPO-Sevilla/Notebook/08_13" title="Go to 13th of August"><span>Go to 13th of August</span></a>
 +
 
 +
    <a class="next_button" href="/Team:UPO-Sevilla/Notebook/09_02" title="Go to 2nd of September"><span>Go to 2nd of September</span></a>
 +
 
 +
    <div class="clear"></div>   
    
    
     <a class="return_button" href="/Team:UPO-Sevilla/Notebook" title="Notebook"><span>Return to Notebook</span></a>
     <a class="return_button" href="/Team:UPO-Sevilla/Notebook" title="Notebook"><span>Return to Notebook</span></a>

Revision as of 12:17, 24 October 2010

September, 01st

Production Team

David Caballero. We wrote the protocol for fecI-fecR* (UPO28*) SDM. It was necessary to remove a PstI site in 1184pb (inside of fecR* secuence). We were going to performance two strategies:

  • fecI-fecR* SDM to get a composed part.
  • fecR* SDM to get only a fecR part. We already had fecI part.

It was not necessary to mutagenize fecA* (UPO8*) or gltD** (UPO17) since we were not going to keep on building devices of them.

Assembly Team

We analyzed all the information we had about parts and devices and toke a decision: we were going to do without device 2 (Fec system) and devices 10, 11 and 12 (Glutamate Synthase). The explication was:

  • D2 was a control of the sensing system. Moreover we could not synthesize fecA part.
  • D10, D11 and D12: glutamate synthase subunits were pretty big so they could give us problems. Also we had problems to remove EcoRI target in one of them by SDM. It was not a huge deal not to continue with that chemoattractant synthesize system because we had other two: aspartate to E. coli and salicilate to P. putida.

We wrote protocols for next devices, SDM of UPO28 and digestion analysis of 1+19 and 16+3.

Go to 13th of August Go to 2nd of September
Return to Notebook

Footer