Team:UPO-Sevilla/Notebook/08 11

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       <h2>Assembly Team</h2>
       <h2>Assembly Team</h2>
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       <p>That day we got a lot of colonies red and white in at least every plaque; non in: 12+19, 11+2+13+3 and 1+2+16+3 plaques. Non DNA controls were cleaned. We made colony PCR with 6 colonies from each plaque. Agarose gel analysis showed than running time was too long. We could not rule out anything, so we set up inocula of each candidate.</p>
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       <p>We had a lot of red and white colonies in every plate; except on: 12+19, 11+2+13+3 and 1+2+16+3 plates. There were no colonies on control plates. We made colony PCR reactions for six colonies from each plate. Agarose gel analysis showed that running time was too long. We could not rule out anything, so we set up inocula of each candidate.</p>
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       <p>We set up inocula of positive candidate from UPO 4, UPO 5 and UPO 11 plates. We made minipreps with the day before inocula and analytic digestions. 0.8% agarosa gel analysis confirm 2+18 and 2+4+2+6.</p>
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       <p>We set up inocula of positive candidate from UPO 4, UPO 5 and UPO 11 plates. We made minipreps and analytic digestions with the day before inocula. 0.8% agarosa gel analysis confirmed 2+18 and 2+4+2+6.</p>
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       <p>We made colony PCR using colonies from 18+3 and 2+6+2+5+3 plates: no positive results.</p>
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       <p>We made colony PCR by using colonies from 18+3 and 2+6+2+5+3 plates: no positive results.</p>

Latest revision as of 14:55, 27 October 2010

August, 11th

Assembly Team

We had a lot of red and white colonies in every plate; except on: 12+19, 11+2+13+3 and 1+2+16+3 plates. There were no colonies on control plates. We made colony PCR reactions for six colonies from each plate. Agarose gel analysis showed that running time was too long. We could not rule out anything, so we set up inocula of each candidate.

We set up inocula of positive candidate from UPO 4, UPO 5 and UPO 11 plates. We made minipreps and analytic digestions with the day before inocula. 0.8% agarosa gel analysis confirmed 2+18 and 2+4+2+6.

We made colony PCR by using colonies from 18+3 and 2+6+2+5+3 plates: no positive results.

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