Team:UPO-Sevilla/Notebook/08 05


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August, 5th

Production Team

Paola Gallardo. We only found white colonies in fecI-fecR plates, and no colonies in control plates. We made a colony PCR of five colonies, but we got negative results. ¿Why? We used lineal vectors. It had no sense. We set up inocula of the same colonies.

David Caballero. Going on with assembly team work, we purified plasmids with new devices. Next, plasmid minipreps were digested using EcoRI and PstI enzymes. Agarose gel electrophoresis analysis showed some positive results: 12+2, 11+2, 7+2. Moreover UPO6 and UPO7 parts were included in pSB1C3 plasmid to send to the Registry of Standard Biological Parts. UPO6 and UPO7 were synthesized using MrGene services.

Assembly Team

And again, and again, and again... colony PCR of UPO 2+6 and 2+7. We have a positive candidate: 2+6! We test the digestion of Mr.Gene biobricks: everything is ok. UPO1+2+4 plate only has red colonies; we repeat this device: ligation and transformation. UPO2+5+3 plate has 5 positive candidates: inocula of these.

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