Team:UPO-Sevilla/Notebook/08 03


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August, 3rd

Production Team

Paola Gallardo. We digest fecI-fecR with restriction enzymes PstI and EcoRI, and run an electrophoresis. We purified from electrophoresis gel (1'5 pDC) and ligate it with the vector C3L (chloramphenicol).

Assembly Team

Again colony PCR of six candidates: 2+4, 2+5 y 2+7. We have positive candidates of UPO2+4 and UPO2+5. We set up inocula of this candidates and spread in LB+Tc plates.

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