Team:UPO-Sevilla/Notebook/08 03

From 2010.igem.org

(Difference between revisions)
Line 24: Line 24:
       <p>Again colony PCR of six candidates: 2+4, 2+5 y 2+7. We have positive candidates of UPO2+4 and UPO2+5. We set up inocula of this candidates and spread in LB+Tc plates.</p>
       <p>Again colony PCR of six candidates: 2+4, 2+5 y 2+7. We have positive candidates of UPO2+4 and UPO2+5. We set up inocula of this candidates and spread in LB+Tc plates.</p>
 +
 +
    <a class="return_button" href="/Team:UPO-Sevilla/Notebook/08_02" title="Go to 2nd of August"><span>Go to 2nd of August</span></a>
 +
 +
    <a class="next_button" href="/Team:UPO-Sevilla/Notebook/08_04" title="Go to 4th of August"><span>Go to 4th of August</span></a>
 +
 +
    <div class="clear"></div>
     <a class="return_button" href="/Team:UPO-Sevilla/Notebook" title="Notebook"><span>Return to Notebook</span></a>
     <a class="return_button" href="/Team:UPO-Sevilla/Notebook" title="Notebook"><span>Return to Notebook</span></a>

Revision as of 12:09, 24 October 2010

August, 3rd

Production Team

Paola Gallardo. We digest fecI-fecR with restriction enzymes PstI and EcoRI, and run an electrophoresis. We purified from electrophoresis gel (1'5 pDC) and ligate it with the vector C3L (chloramphenicol).

Assembly Team

Again colony PCR of six candidates: 2+4, 2+5 y 2+7. We have positive candidates of UPO2+4 and UPO2+5. We set up inocula of this candidates and spread in LB+Tc plates.

Go to 2nd of August Go to 4th of August
Return to Notebook

Footer