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Team:UPO-Sevilla/Notebook/08 03
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<h2>Production Team</h2> | <h2>Production Team</h2> | ||
| - | <p | + | <p>We digest <i>fecI-fecR</i> with PstI and EcoRI restriction enzymes , and run an electrophoresis. We purified from electrophoresis gel (1'5 pb) and ligate it with the vector C3L (chloramphenicol).</p> |
<h2>Assembly Team</h2> | <h2>Assembly Team</h2> | ||
| - | <p> | + | <p>We analyzed several candidates from 2+4, 2+5 and 2+7 plates by colony PCR. We had positive candidates of UPO2+4 and UPO2+5. We set up inocula of these candidates and spread on LB+Tc plates.</p> |
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| + | <a class="return_button" href="/Team:UPO-Sevilla/Notebook/08_02" title="Go to 2nd of August"><span>Go to 2nd of August</span></a> | ||
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| + | <a class="next_button" href="/Team:UPO-Sevilla/Notebook/08_04" title="Go to 4th of August"><span>Go to 4th of August</span></a> | ||
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| + | <div class="clear"></div> | ||
<a class="return_button" href="/Team:UPO-Sevilla/Notebook" title="Notebook"><span>Return to Notebook</span></a> | <a class="return_button" href="/Team:UPO-Sevilla/Notebook" title="Notebook"><span>Return to Notebook</span></a> | ||
Latest revision as of 14:45, 27 October 2010
August, 3rd
Production Team
We digest fecI-fecR with PstI and EcoRI restriction enzymes , and run an electrophoresis. We purified from electrophoresis gel (1'5 pb) and ligate it with the vector C3L (chloramphenicol).
Assembly Team
We analyzed several candidates from 2+4, 2+5 and 2+7 plates by colony PCR. We had positive candidates of UPO2+4 and UPO2+5. We set up inocula of these candidates and spread on LB+Tc plates.
Go to 2nd of August Go to 4th of August Return to Notebook
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