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Team:UPO-Sevilla/Notebook/08 03
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Revision as of 17:11, 13 October 2010
August, 3rd
Production Team
Paola Gallardo. We digest fecI-fecR with restriction enzymes PstI and EcoRI, and run an electrophoresis. We purified from electrophoresis gel (1'5 pDC) and ligate it with the vector C3L (chloramphenicol).
Assembly Team
Again colony PCR of six candidates: 2+4, 2+5 y 2+7. We have positive candidates of UPO2+4 and UPO2+5. We set up inocula of this candidates and spread in LB+Tc plates.
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