"
Team:UPO-Sevilla/Notebook/07 22
From 2010.igem.org
(Difference between revisions)
Lepavgom (Talk | contribs)
(New page: <div class=globalBC> {{:Team:UPO-Sevilla/header}} <!-- --> <html> <head> <script type="text/javascript" language="javascript"> <!-- current("notebook","http...)
Newer edit →
(New page: <div class=globalBC> {{:Team:UPO-Sevilla/header}} <!-- --> <html> <head> <script type="text/javascript" language="javascript"> <!-- current("notebook","http...)
Newer edit →
Revision as of 16:35, 13 October 2010
July, 22th
Production Team
Paola Gallardo. Site-directed mutagenesis (SDM): First try with gltD** and fecI-fecR*, using an overlapping PCR reaction. We analyzed the products of the first round. Fail. We'll try again
David Caballero. Transformation of competent bacteria with ligation products we made the day before. We spread them in LB+Cm plates and let them to grow up overnight at 37ºC.
Assembly Team
UPO1+2 was analized by 1.5% agarose gel electrophoresis again. Results showed three bands with the same size, so our candidate is negative. We started again with UPO1+2: digesting, binding, transforming.
Return to Notebook
Sponsors
