Team:UNIPV-Pavia/Parts/Characterization/ExistingPartsRegistry

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(BBa_K125500 - chloramphenicol resistance cassette)
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=<partinfo>BBa_K125500</partinfo> - GFP fusion brick=
=<partinfo>BBa_K125500</partinfo> - GFP fusion brick=
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This part can be useful to construct fluorescent fusion proteins. It is composed by a tail domain (the GFP <partinfo>K125500</partinfo>) with a transcriptional terminator (<partinfo>BBa_B0015</partinfo>) downstream.
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Other protein domains can be fused upstream of this part in order to create chimeric fluorescent proteins, or it can be ligated to tags useful for low-cost protein purification.
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This part was used do design the following BioBrick measurement systems:
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*<partinfo>BBa_K300086</partinfo>
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*<partinfo>BBa_K300088</partinfo>
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*<partinfo>BBa_K300090</partinfo>
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*<partinfo>BBa_K300091</partinfo>
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*<partinfo>BBa_K300092</partinfo>
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*<partinfo>BBa_K300099</partinfo>
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to test these contructs:
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*<partinfo>BBa_K300002</partinfo>
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*<partinfo>BBa_K300093</partinfo>
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*<partinfo>BBa_K300094</partinfo>
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*<partinfo>BBa_K300095</partinfo>
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*<partinfo>BBa_K300084</partinfo>
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*<partinfo>BBa_K300097</partinfo>
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respectively. All of the tested parts are synthetic fusion tags whose activity could be measured by assembling a promoter with RBS upstream and a tail domain with terminator downstream, thus yielding the measurement systems. <partinfo>BBa_K300005</partinfo> was used as a tail domain with terminator downstream. It has been assembled to test the correct folding of the resulting fusion protein by measuring the GFP and to test the affinity tag performance with a proof of concept protein.
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In all of the measurement parts, GFP could be successfully detected in bacteria harbouring the measurement parts in high copy plasmids. In this condition, GFP was detected by using an excitation filter at 485nm and an emission filter at 540nm in a Infinite F200 microplate reader (Tecan).
=<partinfo>BBa_J72008</partinfo> - phi80 integration helper plasmid pInt80-649=
=<partinfo>BBa_J72008</partinfo> - phi80 integration helper plasmid pInt80-649=
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Revision as of 18:22, 27 October 2010

EXISTING PARTS FROM THE REGISTRY


Return to Characterization

New Parts

Improved Parts

Existing Parts from the Registry


Existing Parts from the Registry: list




BBa_K300009/BBa_I4102 - PoPS->3OC6HSL sender device

BBa_F2620 - 3OC6HSL -> PoPS Receiver

BBa_J61001 - R6K Origin of replication

BBa_J23100, BBa_J23101, BBa_J23105, BBa_J23106, BBa_J23110, BBa_J23114, BBa_J23116, BBa_J23118 - constitutive promoters from Anderson's collection

BBa_P1004 - chloramphenicol resistance cassette

BBa_K125500 - GFP fusion brick

This part can be useful to construct fluorescent fusion proteins. It is composed by a tail domain (the GFP BBa_K125500) with a transcriptional terminator (BBa_B0015) downstream.

Other protein domains can be fused upstream of this part in order to create chimeric fluorescent proteins, or it can be ligated to tags useful for low-cost protein purification.

This part was used do design the following BioBrick measurement systems:

to test these contructs:

respectively. All of the tested parts are synthetic fusion tags whose activity could be measured by assembling a promoter with RBS upstream and a tail domain with terminator downstream, thus yielding the measurement systems. BBa_K300005 was used as a tail domain with terminator downstream. It has been assembled to test the correct folding of the resulting fusion protein by measuring the GFP and to test the affinity tag performance with a proof of concept protein.


In all of the measurement parts, GFP could be successfully detected in bacteria harbouring the measurement parts in high copy plasmids. In this condition, GFP was detected by using an excitation filter at 485nm and an emission filter at 540nm in a Infinite F200 microplate reader (Tecan).

BBa_J72008 - phi80 integration helper plasmid pInt80-649