Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test27settembre
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==RESULTS== | ==RESULTS== | ||
- | [[Image:UNIPV_Pavia_ASB27settembre.png|thumb|500px |center| | + | [[Image:UNIPV_Pavia_ASB27settembre.png|thumb|500px |center|Raw growth curve]] |
- | [[Image:UNIPV_Pavia_GFP27settembre.png|thumb|500px |center|GFP curve]] | + | [[Image:UNIPV_Pavia_GFP27settembre.png|thumb|500px |center|Raw GFP curve]] |
[[Image:UNIPV_Pavia_SScellM27settembre.png|thumb|500px |center|Mean of (dGFP/dt)/O.D.600]] | [[Image:UNIPV_Pavia_SScellM27settembre.png|thumb|500px |center|Mean of (dGFP/dt)/O.D.600]] | ||
- | All cell cultures showed a similar growth curve. After an initial interval in witch GFP production rate was significantly higher in positive control <partinfo>BBa_K173000</partinfo> than in the other samples, GFP synthesis rate of I47, I48, I49 became very similar to this one. It was still lower than positive control, but was constant during all the experiment. This showed the right folding of the green fluorescent protein assembled downstream of the genetic circuit. | + | All cell cultures showed a similar growth curve and doubling time was computed as described [[Team:UNIPV-Pavia/Parts/Characterization#Doubling_time_evaluation|here]] in order to have informations about the burden due to synthesis of such fusion proteins. It's possible to see |
+ | In GFP curve it's possible to appreciate that in I47, I48, I49 GFP accumulation it's very similar and it's significantly different from that of negative control <partinfo>BBa_B0031</partinfo>. | ||
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+ | After an initial interval in witch GFP production rate was significantly higher in positive control <partinfo>BBa_K173000</partinfo> than in the other samples, GFP synthesis rate of I47, I48, I49 became very similar to this one. It was still lower than positive control, but was constant during all the experiment. This showed the right folding of the green fluorescent protein assembled downstream of the genetic circuit. | ||
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Revision as of 22:50, 23 October 2010
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