Team:UNIPV-Pavia/Calendar/October/settimana2

From 2010.igem.org

(Difference between revisions)
(October, 12th)
(October, 12th)
Line 62: Line 62:
-BBa_K300977, BBa_K300978 (P3-P4).
-BBa_K300977, BBa_K300978 (P3-P4).
-
[[Image:UNIPV1013_10_10_mcflippati(p1p4)_mgflippati(p1p4)_mcflippati(p3p4) _mgflippati(p3p4).jpg|thumb|300px|center|The order of the samples were the follow: MC43_flip(A,B,C), MG43_flip(A,B,C), Cneg (MG1655), blank. Primers were (from left to right) BBa_K300975-BBa_K300978, BBa_K300977-BBa_K300978.]]
+
[[Image:UNIPV1013_10_10_mcflippati(p1p4)_mgflippati(p1p4)_mcflippati(p3p4) _mgflippati(p3p4).jpg|thumb|300px|center|The order of the samples was the follow: MC43_flip(A,B,C), MG43_flip(A,B,C), Cneg (MG1655), blank. Primers were (from left to right) BBa_K300975-BBa_K300978, BBa_K300977-BBa_K300978.]]
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[[Image:UNIPV10_13-10-10_mcflippati(vf2-vr)_mgflippati(vf2-vr)_cneg_blank.jpg|thumb|300px|center|The order of the samples were the follow: MC43_flip(A,B,C), MG43_flip(A,B,C), Cneg (MG1655), blank. Primers were (from left to right) BBa_G00100-BBa_G00101.]]
+
[[Image:UNIPV10_13-10-10_mcflippati(vf2-vr)_mgflippati(vf2-vr)_cneg_blank.jpg|thumb|300px|center|The order of the samples was the follow: MC43_flip(A,B,C), MG43_flip(A,B,C), Cneg (MG1655), blank. Primers were (from left to right) BBa_G00100-BBa_G00101.]]
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For unknown reasons MC43_C didn't show any bands so we decided to perform again the screening with the same primers. At the same time we performed the screening PCR on the two negative controls with the primers BBa_K300977-BBa_K300978 and BBa_G00100-BBa_G00101. The results were the follows:
 +
 
 +
[[Image:UNIPV10_14_10_10_PCR_Cneg(p3p4)_blank_MC43Cflip(LB-M9)(p1p4)_blank_Cneg(vf2vr)_blank.jpg|thumb|300px|center|The order of the samples was the follow: MG1655 (from 2 to 5) with BBa_K300977-BBa_K300978 primers, blank, MC43_flipC-1, MC43_flipC-2 with primers BBa_K300975-BBa_K300978, blank and MC1061 (from 10 to 13) with primers BBa_G00100-BBa_G00101]]
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<font color='red' size='+2'>We are now moving all our parts to <partinfo>pSB1C3</partinfo> plasmid</font>
<font color='red' size='+2'>We are now moving all our parts to <partinfo>pSB1C3</partinfo> plasmid</font>

Revision as of 15:20, 22 October 2010


OCTOBER: WEEK 2



October, 11th


We are now moving all our parts to <partinfo>pSB1C3</partinfo> plasmid


Inoculum of MC42-A, MC43-A, MG42-B, MG43-A, MC43_flip-a,b,c, MG43_flip-a,b,c, MG1655.

October, 12th

Screening PCR was performed on MC42, MG42, MC43, MG43 and MG1655 with the following primers:

-BBa_K300975, BBa_K300976 (P1-P2)

-BBa_K300976, BBa_K300977 (P2-P3)

-BBa_K300977, BBa_K300978 (P3-P4).

The order of the samples were the follow: MC42, MC43, MG42, MG43, Cneg (MG1655), blank. Primers were (from left to right) BBa_K300975-BBa_K300976, BBa_K300976-BBa_K300977, BBa_K300977-BBa_K300978.

Screening PCR was performed on MC43_flip(A,B,C), MG43_flip(A,B,C) and MG1655 with the following primers:

-BBa_K300975, BBa_K300978 (P1-P4)

-BBa_G00100,BBa_G00101 (VF2-VR)

-BBa_K300977, BBa_K300978 (P3-P4).

The order of the samples was the follow: MC43_flip(A,B,C), MG43_flip(A,B,C), Cneg (MG1655), blank. Primers were (from left to right) BBa_K300975-BBa_K300978, BBa_K300977-BBa_K300978.
The order of the samples was the follow: MC43_flip(A,B,C), MG43_flip(A,B,C), Cneg (MG1655), blank. Primers were (from left to right) BBa_G00100-BBa_G00101.

For unknown reasons MC43_C didn't show any bands so we decided to perform again the screening with the same primers. At the same time we performed the screening PCR on the two negative controls with the primers BBa_K300977-BBa_K300978 and BBa_G00100-BBa_G00101. The results were the follows:

The order of the samples was the follow: MG1655 (from 2 to 5) with BBa_K300977-BBa_K300978 primers, blank, MC43_flipC-1, MC43_flipC-2 with primers BBa_K300975-BBa_K300978, blank and MC1061 (from 10 to 13) with primers BBa_G00100-BBa_G00101

We are now moving all our parts to <partinfo>pSB1C3</partinfo> plasmid


October, 13th


We are now moving all our parts to <partinfo>pSB1C3</partinfo> plasmid


October, 14th


We are now moving all our parts to <partinfo>pSB1C3</partinfo> plasmid


October, 15th


We are now moving all our parts to <partinfo>pSB1C3</partinfo> plasmid


October, 16th


We are now moving all our parts to <partinfo>pSB1C3</partinfo> plasmid