Team:UNIPV-Pavia/Calendar/June

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Revision as of 17:52, 7 June 2010

JUNE

DATE	ACTIVITY
June, 3th	9° Meeting - Discussion of final and technical details about The Project. Planning of Bio-Lab activity.
June, 7th	1° Bio-Lab - Susanna, Nicolò and Federica. <partinfo>BBa_E2050</partinfo>, <partinfo>BBa_K165018</partinfo>, <partinfo>BBa_K165037</partinfo>, <partinfo>BBa_J61001</partinfo>, <partinfo>BBa_K081008</partinfo> and <partinfo>BBa_K125500</partinfo> were resuspended from Spring 2010 DNA distribution. <partinfo>BBa_P1004</partinfo> was resuspended from Spring 2009 DNA distribution. All BioBricks were tranformed in E. coli DH5alpha Liquid LB+Amp and LB agar plates +Amp were prepared according with "protocols".
June, 8th	2° Bio-Lab - Susanna, Alessandro and Chiara.
June, 9th	3° Bio-Lab - Susanna, Nicolò and Sara.
June, 10th	4° Bio-Lab - Susanna, Manuel Z. and Riccardo.
June, 11th	5° Bio-Lab - Susanna and Nicolò.

June, 7th

These BioBrick were resuspended in 15ul ddH20:

BBa_E2050 (mOrange, 744bp) 2010 Kit Plate 2, well 13N in pSB2K3
BBa_K165018 (ADH1 terminator, 253 bp) 2010 Kit Plate 3, well 2M, J63009 (Amp)
BBa_K165037 (tef2 promoter, 403bp) 2010 Kit Plate 3, well 22O, pSB1AK3
BBa_P1004 (Chloramphenicol resistence cassette, 769 bp) 2009 Kit plate 1, well 7B, pSB1A1
BBa_J61001 (R6K origin, 406bp) 2010 Kit plate 1, well 240, pSB1A2
BBa_K081008 (RBS-luxI, 664bp) 2010 Kit plate 2, well 10L, pSB1A2
BBa_K125500 (GFP fusion brick, 718bp) 2010 Kit plate 3, well 2P, pSB1A2

1,5 ul of each culture was transformed in 100 ul of home-made competent E. coli DH5alpha.

Tranforants were all plated on LB agar plate added with Ampicillin, except for BBa_E2050 (Kanamycin).

500 ml LB+Amp was prepared and 21 LB agar plates + Amp were prepared (500 ml).

@@ Line 14: / Line 14: @@
 <tr><th align="center" width="12%"><b>DATE</b></th><th align="center"><b>ACTIVITY</b></th></tr>
 <tr><td align="center" valign="top">June, 3th</td><td align="left">9° Meeting - Discussion of final and technical details about [[Team:UNIPV-Pavia/Project|The Project]]. Planning of Bio-Lab activity.</td></tr>
-<tr><td align="center" valign="top">[[#June, 7th|June, 7th]]</td><td align="left">1° Bio-Lab - Susanna, Nicolò and Federica. <partinfo>BBa_E2050</partinfo>,<partinfo>BBa_K165018</partinfo>, <partinfo>BBa_K165037</partinfo>, <partinfo>BBa_J61001</partinfo>, <partinfo>BBa_K081008</partinfo> and <partinfo>BBa_K125500</partinfo> were resuspended from Spring 2010 DNA distribution. <partinfo>BBa_P1004</partinfo> was resuspended from Spring 2009 DNA distribution. All BioBricks were tranformed in E. coli DH5alpha<br>Liquid LB and LB agar plates were prepared according with "protocols"</td></tr>
+<tr><td align="center" valign="top">[[#June, 7th|June, 7th]]</td><td align="left">1° Bio-Lab - Susanna, Nicolò and Federica. <partinfo>BBa_E2050</partinfo>, <partinfo>BBa_K165018</partinfo>, <partinfo>BBa_K165037</partinfo>, <partinfo>BBa_J61001</partinfo>, <partinfo>BBa_K081008</partinfo> and <partinfo>BBa_K125500</partinfo> were resuspended from Spring 2010 DNA distribution. <partinfo>BBa_P1004</partinfo> was resuspended from Spring 2009 DNA distribution. All BioBricks were tranformed in E. coli DH5alpha<br>Liquid LB+Amp and LB agar plates +Amp were prepared according with "protocols".</td></tr>
 <tr><td align="center" valign="top">June, 8th</td><td align="left">2° Bio-Lab - Susanna, Alessandro and Chiara.<br></td></tr>
 <tr><td align="center" valign="top">June, 9th</td><td align="left">3° Bio-Lab - Susanna, Nicolò and Sara.<br></td></tr>