Team:UNIPV-Pavia/Calendar/August/settimana4
From 2010.igem.org
m (→August, 25th) |
m (→August, 25th) |
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*I40-7: | *I40-7: | ||
- | Samples were digested E-P for three hours | + | Samples were digested E-P for three hours |
{| border="1" align='center' | {| border="1" align='center' | ||
| ''Culture'' || ''Kind'' || ''Final reaction volume (ul) '' || ''DNA (ul)'' || ''H20 (ul)'' || ''Enzyme 1'' || ''Enzyme 2'' || ''Buffer H'' | | ''Culture'' || ''Kind'' || ''Final reaction volume (ul) '' || ''DNA (ul)'' || ''H20 (ul)'' || ''Enzyme 1'' || ''Enzyme 2'' || ''Buffer H'' | ||
Line 73: | Line 73: | ||
| I40-7 || Insert/Screening || 25 || x || x || 1 EcoRI || 1 PstI || 2,5 | | I40-7 || Insert/Screening || 25 || x || x || 1 EcoRI || 1 PstI || 2,5 | ||
|} | |} | ||
+ | and than gel run to check the length of ligations. | ||
[[Image:UNIPV10_25_8_10_E-PscreeningI40.jpg|thumb|300px|center|Gel run for I40-1/4/5/6/7 digested E-P]] | [[Image:UNIPV10_25_8_10_E-PscreeningI40.jpg|thumb|300px|center|Gel run for I40-1/4/5/6/7 digested E-P]] | ||
As you can see ... ,so we decided to keep glycerol stock of I40-X. | As you can see ... ,so we decided to keep glycerol stock of I40-X. |
Revision as of 19:43, 24 August 2010
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